Yasumizu R, Hiai H, Sugiura K, Oyaizu N, Fan H X, Ohnishi Y, Iwai H, Inaba M, Kakinuma M, Onoe K
Department of Pathology, Kansai Medical University, Osaka, Japan.
J Immunol. 1988 Sep 15;141(6):2181-6.
The transplantation of bone marrow cells from BALB/c (but not C57BL/6 and C3H/HeN) mice was observed to lead to the development of thymic lymphomas (leukemias) in AKR/J mice. Two leukemic cell lines, CAK1.3 and CAK4.4, were established from the primary culture of two thymic lymphoma, and surface phenotypes of these cell lines found to be H-2d and Thy-1.2+, indicating that these lymphoma cells are derived from BALB/c donor bone marrow cells. Further analyses of surface markers revealed that CAK1.3 is L3T4+ Lyt2+ IL2R-, whereas CAK4.4 is L3T4- Lyt2- IL2R+. Both CAK1.3 and CAK4.4 were transplantable into BALB/c but not AKR/J mice, further indicating that these cells are of BALB/c bone marrow donor origin. The cells were found to produce XC+-ecotropic viruses, but xenotropic and mink cell focus-forming viruses were undetectable. Inasmuch as thymic lymphomas are derived from bone marrow cells of leukemia-resistant BALB/c strain of mice under the allogeneic environment of leukemia-prone AKR/J mice, this animal model may serve as a useful tool not only for the analysis of leukemic relapse after bone marrow transplantation but also for elucidation of the mechanism of leukemogenesis.
观察发现，将BALB/c小鼠（而非C57BL/6和C3H/HeN小鼠）的骨髓细胞移植到AKR/J小鼠体内会导致胸腺淋巴瘤（白血病）的发生。从两例胸腺淋巴瘤的原代培养物中建立了两个白血病细胞系CAK1.3和CAK4.4，发现这些细胞系的表面表型为H-2d和Thy-1.2+，表明这些淋巴瘤细胞来源于BALB/c供体骨髓细胞。对表面标志物的进一步分析显示，CAK1.3为L3T4+ Lyt2+ IL2R-，而CAK4.4为L3T4- Lyt2- IL2R+。CAK1.3和CAK4.4均可移植到BALB/c小鼠体内，但不能移植到AKR/J小鼠体内，这进一步表明这些细胞来源于BALB/c骨髓供体。发现这些细胞可产生XC+嗜亲性病毒，但未检测到异嗜性病毒和貂细胞集落形成病毒。鉴于胸腺淋巴瘤是在白血病易感的AKR/J小鼠的同种异体环境下，由白血病抗性BALB/c品系小鼠的骨髓细胞产生的，该动物模型不仅可作为分析骨髓移植后白血病复发的有用工具，还可用于阐明白血病发生的机制。
