Dickinson J R, Hewlins M J
Department of Microbiology, University College, Cardiff, UK.
J Gen Microbiol. 1988 Feb;134(2):333-7. doi: 10.1099/00221287-134-2-333.
13C NMR was used to study the pattern of label incorporation from [2-13C]acetate into trehalose during sporulation in Saccharomyces cerevisiae. A wild-type strain and a strain homozygous for the zwf1 mutation (which affects glucose-6-phosphate dehydrogenase) were used. In the wild-type it was possible to deduce the cycling of glucose 6-phosphate around the hexose monophosphate pathway whilst in the mutant strain this did not occur. The requirement of the hexose monophosphate pathway for providing NADPH for fatty acid biosynthesis was examined using 13C NMR and GC/MS. The wild-type strain produced a typical profile of fatty acids with palmitoleic acid being the most abundant whereas the mutant contained only one-quarter the amount of total fatty acid. As zwf1 homozygous diploids are able to sporulate this indicates that the large amount of fatty acid biosynthesis observed in sporulation of wild-type strains is not essential to the process.
采用¹³C核磁共振技术研究酿酒酵母在孢子形成过程中[2-¹³C]乙酸盐中标记物掺入海藻糖的模式。使用了野生型菌株和zwf1突变纯合菌株（该突变影响葡萄糖-6-磷酸脱氢酶）。在野生型菌株中，可以推断出6-磷酸葡萄糖在磷酸己糖途径中循环，而在突变菌株中则未发生这种情况。使用¹³C核磁共振和气相色谱/质谱联用技术研究了磷酸己糖途径为脂肪酸生物合成提供NADPH的必要性。野生型菌株产生了典型的脂肪酸谱，其中棕榈油酸含量最高，而突变体中总脂肪酸含量仅为野生型的四分之一。由于zwf1纯合二倍体能够形成孢子，这表明在野生型菌株孢子形成过程中观察到的大量脂肪酸生物合成对该过程并非必不可少。
