Dickinson J R, Williams A S
J Gen Microbiol. 1986 Sep;132(9):2605-10. doi: 10.1099/00221287-132-9-2605.
The requirement for gluconeogenesis and the pentose phosphate pathway in sporulation of Saccharomyces cerevisiae was investigated using homozygous diploids with mutations in selected portions of the respective metabolic pathways. Mutations affecting the genes FBA1 (fructose-1,6-bisphosphate aldolase), GPM1 (phosphoglycerate mutase) and ZWF1 (glucose-6-phosphate dehydrogenase) were used. Homozygous diploids bearing either fba1-11 or gpm1 mutations were asporogenous, indicating an absolute requirement for gluconeogenesis in sporulation. A strain homozygous for the zwf1 mutation sporulated, but at a reduced level compared to the wild-type. Homozygous spd1-1 mutations restored the ability to sporulate in fba1-11 homozygous diploids; this is believed to occur as a consequence of reduced NH+4 levels in spd1-1-bearing strains, the reduced intracellular NH+4 content serving to promote gluconeogenesis via the residual low levels of enzyme activity present in such mutants.
利用在各自代谢途径特定部分发生突变的纯合二倍体，研究了酿酒酵母孢子形成过程中对糖异生作用和磷酸戊糖途径的需求。使用了影响FBA1（果糖-1,6-二磷酸醛缩酶）、GPM1（磷酸甘油酸变位酶）和ZWF1（葡萄糖-6-磷酸脱氢酶）基因的突变。携带fba1-11或gpm1突变的纯合二倍体不产孢子，这表明孢子形成过程中对糖异生作用有绝对需求。一个zwf1突变纯合的菌株能够形成孢子，但与野生型相比水平降低。纯合的spd1-1突变恢复了fba1-11纯合二倍体的孢子形成能力；据信这是由于携带spd1-1的菌株中NH+4水平降低所致，细胞内NH+4含量降低通过此类突变体中残留的低水平酶活性促进了糖异生作用。
