Yli-Mattila Tapani, Hussien Taha, Gavrilova Olga, Gagkaeva Tatiana
Department of Biochemistry, University of Turku, Turku 20014, Finland.
National Research Center, Cairo 80301, Egypt.
Pathogens. 2018 Nov 29;7(4):94. doi: 10.3390/pathogens7040094.
and closely related species are common fungi on various plants, cultivated in different climatic regions. The aim of this study was to determine the taxonomic affiliations of the , and strains by using morphological, physiological and molecular-genetic approaches. Twenty-six single-spored morphologically identified strains, which were mainly from cereals, were investigated in order to find out, if they belong to a separate species. Pathogenicity of strains to wheat seedlings and ISSR (Inter Simple Sequence Repeats) fingerprint and beta-tubulin DNA sequence patterns were analyzed. According to phylogenetic analyses, the strains could be divided into two big groups consisting of mostly or strains. was not detected as a separate species by the sum of the characters. was characterized as a separate species, which could be identified by morphological and molecular data. High genetic diversity of the and related species was revealed. One strain (rudbeckia, Vladivostok, Russia), had an identical beta-tubulin sequence with two previously sequenced strains of species complex, which were isolated from dicotyledonous plants in Asia.
与其亲缘关系密切的物种是各种植物上常见的真菌,生长于不同气候区域。本研究的目的是通过形态学、生理学和分子遗传学方法确定[具体物种名称1]、[具体物种名称2]和[具体物种名称3]菌株的分类归属。对26个主要来自谷物的经形态学鉴定的单孢菌株进行了研究,以确定它们是否属于一个独立的物种。分析了这些菌株对小麦幼苗的致病性以及ISSR(简单重复序列区间)指纹图谱和β-微管蛋白DNA序列模式。根据系统发育分析,这些菌株可分为两大类,主要由[具体物种名称1]或[具体物种名称2]菌株组成。综合各项特征未检测到[具体物种名称3]为一个独立的物种。[具体物种名称2]被鉴定为一个独立的物种,可通过形态学和分子数据进行识别。揭示了[具体物种名称1]及其相关物种的高度遗传多样性。一个[具体物种名称1]菌株(来自俄罗斯符拉迪沃斯托克的金光菊)与两个先前测序的[具体物种名称2]复合种菌株具有相同的β-微管蛋白序列,这两个菌株是从亚洲双子叶植物中分离得到的。