Biocomplexity Institute, Indiana University, Bloomington, IN, USA; Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.
Biocomplexity Institute, Indiana University, Bloomington, IN, USA; Department of Physics, Indiana University, Bloomington, IN, USA.
Microvasc Res. 2019 May;123:7-13. doi: 10.1016/j.mvr.2018.11.010. Epub 2018 Nov 28.
Microvascular perfusion dynamics are vital to physiological function and are frequently dysregulated in injury and disease. Typically studies measure microvascular flow in a few selected vascular segments over limited time, failing to capture spatial and temporal variability. To quantify microvascular flow in a more complete and unbiased way we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow), a macro for FIJI open-source image analysis software. Using high-speed microvascular flow movies, STAFF generates kymographs for every time interval for every vascular segment, calculates flow velocities from red blood cell shadow angles, and outputs the data as color-coded velocity map movies and spreadsheets. In untreated mice, analyses demonstrated profound variation even between adjacent sinusoids over seconds. In acetaminophen-treated mice we detected flow reduction localized to pericentral regions. STAFF is a powerful new tool capable of providing novel insights by enabling measurement of the complex spatiotemporal dynamics of microvascular flow.
微血管灌注动力学对于生理功能至关重要，在损伤和疾病中经常失调。通常，研究在有限的时间内测量少数选定的血管段中的微血管流量，无法捕捉到空间和时间的可变性。为了更全面和无偏地量化微血管流量，我们开发了 STAFF（现场流量的时空分析），这是 FIJI 开源图像分析软件的一个宏。使用高速微血管流量电影，STAFF 为每个血管段的每个时间间隔生成动图，从红细胞阴影角度计算流速，并将数据输出为彩色编码速度图电影和电子表格。在未处理的小鼠中，分析表明，即使在相邻的窦之间，几秒钟内也会发生明显的变化。在对乙酰氨基酚处理的小鼠中，我们检测到局部到中央区域的血流减少。STAFF 是一种强大的新工具，通过能够测量微血管流量的复杂时空动态，提供新的见解。
