Willms Inka M, Hoppert Michael, Hertel Robert
Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of Goettingen, 37077, Goettingen, Germany.
Department of General Microbiology, Institute of Microbiology and Genetics, University of Goettingen, 37077 Goettingen, Germany.
Viruses. 2017 Jun 12;9(6):146. doi: 10.3390/v9060146.
The viruses are ubiquitous in nature and have an obligatory virulent lifestyle. They infect , a bacterial phylum containing an array of environmental non-pathogenic and pathogenic organisms. To expand the knowledge of this viral subfamily, new strains were isolated and investigated in this study. Here we present two new viruses, vB_BsuM-Goe2 and vB_BsuM-Goe3, isolated from raw sewage and infecting species. Both were morphologically classified via transmission electron microscopy (TEM) as members of the subfamily belonging to the family. Genomic sequencing and analyses allowed further affiliation of vB_BsuM-Goe2 to the SPO1-like virus group and vB_BsuM-Goe3 to the Bastille-like virus group. Experimentally determined adsorption constant, latency period, burst size and host range for both viruses revealed different survival strategies. Thus vB_BsuM-Goe2 seemed to rely on fewer host species compared to vB_BsuM-Goe3, but efficiently recruits those. Stability tests pointed out that both viruses are best preserved in LB-medium or TMK-buffer at 4 or 21 °C, whereas cryopreservation strongly reduced viability.
这些病毒在自然界中无处不在，具有一种必然的致病生活方式。它们感染厚壁菌门，该菌门包含一系列环境中的非致病和致病生物。为了扩展对这个病毒亚科的认识，本研究分离并研究了新的菌株。在此，我们展示了两种从未经处理的污水中分离出来并感染芽孢杆菌属物种的新病毒，即vB_BsuM-Goe2和vB_BsuM-Goe3。通过透射电子显微镜（TEM）对两者进行形态学分类，它们均属于芽孢杆菌病毒科芽孢杆菌病毒亚科的成员。基因组测序和分析进一步确定vB_BsuM-Goe2属于SPO1样病毒组，vB_BsuM-Goe3属于巴士底狱样病毒组。对这两种病毒通过实验测定的吸附常数、潜伏期、爆发量和宿主范围揭示了不同的生存策略。因此，与vB_BsuM-Goe3相比，vB_BsuM-Goe2似乎依赖的宿主物种较少，但能有效地利用这些宿主。稳定性测试指出，这两种病毒在4℃或21℃的LB培养基或TMK缓冲液中保存效果最佳，而冷冻保存会大大降低其活力。
