Golomidova Alla K, Kulikov Eugene E, Babenko Vladislav V, Ivanov Pavel A, Prokhorov Nikolai S, Letarov Andrey V
Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences», Winogradsky Institute of Microbiology, prosp. 60-letiya Oktyabrya, 7/2, 117312, Moscow, Russian Federation.
Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, 141701, Moscow, Russian Federation.
Arch Virol. 2019 Mar;164(3):879-884. doi: 10.1007/s00705-018-4113-2. Epub 2018 Dec 1.
Escherichia coli bacteriophage Gostya9 (genus T5virus) was isolated from horse feces collected in Moscow, Russia, in 2013. This phage was associated in a single plaque with the previously reported phage 9g and was subsequently purified. Analysis of the complete genomic sequence of Gostya9 revealed that it is closely related to the T5-like bacteriophage DT57C, which had been isolated at the same location in 2007. These two viruses share 79.5% nucleotide sequence identity, which is below the 95% threshold applied currently to demarcate bacteriophage species. The most significant features distinguishing Gostya9 from DT57C include 1) the presence of one long tail fiber protein gene, 122c (ltf), instead of the two genes, ltfA and ltfB, that are present in DT57C; 2) the absence of the gene for the receptor-blocking lytic conversion lipoprotein precursor llp; and 3) the divergence of the receptor-recognition protein, pb5, which is only distantly related at the amino acid sequence level. The observed features of the Gostya9 adsorption apparatus are suggestive of a possible novel specificity for the final receptor and make this phage interesting for possible direct application in phage therapy of E. coli infections or as a source of receptor-recognition protein for engineering new phage specificities.
大肠杆菌噬菌体Gostya9（T5病毒属）于2013年从俄罗斯莫斯科收集的马粪便中分离得到。该噬菌体与之前报道的噬菌体9g在单个噬菌斑中相关联，随后被纯化。对Gostya9完整基因组序列的分析表明，它与2007年在同一地点分离的T5样噬菌体DT57C密切相关。这两种病毒的核苷酸序列同一性为79.5%，低于目前用于划分噬菌体物种的95%阈值。区分Gostya9与DT57C的最显著特征包括：1）存在一个长尾纤维蛋白基因122c（ltf），而不是DT57C中存在的两个基因ltfA和ltfB；2）不存在受体阻断裂解转化脂蛋白前体llp的基因；3）受体识别蛋白pb5的差异，其在氨基酸序列水平上仅具有远缘关系。观察到的Gostya9吸附装置的特征表明其对最终受体可能具有新的特异性，这使得该噬菌体对于直接应用于大肠杆菌感染的噬菌体治疗或作为工程新噬菌体特异性的受体识别蛋白来源具有吸引力。
