Department of Microbiology, Third Military Medical University, Chongqing, China.
Intervirology. 2010;53(4):211-20. doi: 10.1159/000299063. Epub 2010 Mar 23.
To characterize morphological, physicochemical and genomic features of a novel virulent coliphage which was isolated from an engineered Escherichia coli culture and termed engineered E. coli phage (EEP).
Electron microscopy revealed that EEP has an icosahedral head (62 nm in diameter) and a long, flexible tail (138 nm in length). EEP was able to infect all 10 engineered E. coli strains kept in our laboratory, showing a strong ability to lyse engineered E. coli. Sequencing of the EEP genome revealed a double-stranded DNA (39.8 kb) with 54.72% GC content. Fifty-two open reading frames were predicted to be coding sequences, 18 of which were functionally defined and organized in a modular format, which includes modules for DNA replication, DNA packaging, structural proteins and host cell lysis. This phage could not be inactivated at 90 degrees for 45 min and was resistant to ethanol and alkali treatment. EEP is assigned to the Siphoviridae family based on its morphological, genomic and physicochemical properties.
A novel coliphage was isolated from engineered E. coli strains, and its morphological, genomic and physicochemical properties were characterized, which will improve our knowledge of bacteriophage diversity.
对从工程大肠杆菌培养物中分离出的新型烈性噬菌体进行形态学、理化和基因组特征分析，将其命名为工程大肠杆菌噬菌体（EEP）。
电子显微镜观察发现，EEP 具有二十面体头部（直径 62nm）和长而灵活的尾部（长 138nm）。EEP 能够感染我们实验室保存的所有 10 株工程大肠杆菌菌株，表现出强烈的裂解工程大肠杆菌的能力。EEP 基因组测序显示为双链 DNA（39.8kb），GC 含量为 54.72%。预测有 52 个开放阅读框为编码序列，其中 18 个具有功能定义，并以模块化格式组织，包括 DNA 复制、DNA 包装、结构蛋白和宿主细胞裂解模块。该噬菌体在 90°C 下 45 分钟不能失活，并且对乙醇和碱处理具有抗性。根据其形态学、基因组和理化特性，EEP 被归为肌尾噬菌体科。
从工程大肠杆菌菌株中分离出一种新型噬菌体，并对其形态学、基因组和理化特性进行了表征，这将提高我们对噬菌体多样性的认识。
