Laboratory of Nutrition and Functional Food, Jilin University, 5333 Xi'An Road, Changchun, 130062, People's Republic of China.
Arch Virol. 2013 Nov;158(11):2429-32. doi: 10.1007/s00705-013-1727-2. Epub 2013 Jun 12.
A novel virulent phage named JL1 against Escherichia coli O157:H7 was isolated from raw sewage. It was found that JL1 has an icosahedral head and a long flexible non-contractile tail. The complete genome of JL1 is composed of a linear double-stranded DNA of 43,457 base pairs in length, with 54.77 % G+C content and 60 putative open reading frames. Morphology and bioinformatics analysis revealed that phage JL1 is a member of the family Siphoviridae of the order Caudovirales. It is different from previously reported phages of E. coli O157:H7 but is homologous to Sodalis phage SO-1, Shigella phage EP23, Escherichia phage HK578 and Escherichia phage SSL-2009a.
从污水中分离到一株针对大肠杆菌 O157:H7 的烈性噬菌体 JL1，其头部呈二十面体，尾部为长而柔软的非收缩性。JL1 的全基因组由一个线性双链 DNA 组成，长度为 43457 个碱基对，GC 含量为 54.77%，包含 60 个推定的开放阅读框。形态学和生物信息学分析表明，噬菌体 JL1 属于长尾病毒目 Siphoviridae 科。它与先前报道的大肠杆菌 O157:H7 噬菌体不同，但与 Sodalis 噬菌体 SO-1、志贺氏菌噬菌体 EP23、大肠杆菌噬菌体 HK578 和大肠杆菌噬菌体 SSL-2009a 同源。
