Liu Yi, Mi Bobin, Lv Huijuan, Liu Jing, Xiong Yuan, Hu Liangcong, Xue Hang, Panayi Adriana C, Liu Guohui, Zhou Wu
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.
J Cell Biochem. 2019 May;120(5):7741-7750. doi: 10.1002/jcb.28048. Epub 2018 Dec 3.
The beneficial effects of icariin in the management of many diseases, such as chronic renal failure and heart failure, are well known. Icariin has also been shown to ameliorate osteoarthritis (OA) symptoms; however, the underlying mechanisms remain unclear. In this study, a bioinformatics analysis was performed to investigate the KEGG pathways of icariin-targeted genes involved in OA. Our study suggests that icariin plays a role in OA by regulating inflammatory cytokine production, insulin resistance, and cell survival through modulation of the NF-κB, MAPK, and Akt signaling pathways. Importantly, IKBKB, NFKBIA, MAPK8, MAPK9, and MAPK10 may be the hub genes affected by icariin when providing its beneficial effects on OA. In addition, we found that icariin decreases proinflammatory factors and inhibits chondrocyte apoptosis through suppression of the NF-κB pathway. Our study highlights a set of KEGG pathways that could explain the molecular mechanism of icariin's action on OA, suggesting that icariin could be considered as a promising therapeutic option for OA.
淫羊藿苷在许多疾病（如慢性肾衰竭和心力衰竭）的治疗中所具有的有益作用是众所周知的。淫羊藿苷也已被证明可改善骨关节炎（OA）症状；然而，其潜在机制仍不清楚。在本研究中，进行了一项生物信息学分析，以研究淫羊藿苷靶向的参与OA的基因的KEGG通路。我们的研究表明，淫羊藿苷通过调节炎症细胞因子的产生、胰岛素抵抗以及通过调节NF-κB、MAPK和Akt信号通路来影响细胞存活，从而在OA中发挥作用。重要的是，IKBKB、NFKBIA、MAPK8、MAPK9和MAPK10可能是淫羊藿苷在对OA产生有益作用时所影响的关键基因。此外，我们发现淫羊藿苷通过抑制NF-κB途径降低促炎因子并抑制软骨细胞凋亡。我们的研究突出了一组KEGG通路，这些通路可以解释淫羊藿苷对OA作用的分子机制，表明淫羊藿苷可被视为OA的一种有前景的治疗选择。
