Dosoky Noura S, Satyal Prabodh, Pokharel Suraj, Setzer William N
Nat Prod Commun. 2016 Dec;11(12):1895-1898.
Rhododendron anthopogon D. Don., A small compact Himalayan shrub growing in Nepal, is a known medicinal plant used to treat sore throat, colds, blood disorders, bone disease, potato allergies, and vomiting, and to relieve liver disorders, headaches and back pain. The present study investigated the chemical composition and bioactivities of the leaf essential oil from R. anthopogon from Dhankuta, Nepal. The essential oil from leaves was obtained by hydrodistillation and a detailed chemical analysis was conducted by gas chromatography - mass spectrometry.(GC-MS). The enantiomeric distribution of monoterpenoid components was determined using chiral gas chromatography and represents the first chiral examination of R. anthopogon essential oil. The essential oil was screened for antimicrobial activity using the microbroth dilution test, and for cytotoxic activity against MCF-7, MDA-MB-231, and 5637 using the MTT assay. A total of 70 volatile componeits were identified from the essential oil. The major components were α-pinene (21.5%), δ-cadinene (13.8%), β-pinene (9.5%), limoiene (5.9%), δ-amorphene (4.6%), α-muurolene (4.5%), and (E)-daryophyllene (3.2%) with other minor constituents (< 3%). The essential oil showed marginal antibacterial and.cytotoxic activities, but no antifungal effects.
髯花杜鹃（Rhododendron anthopogon D. Don.）是一种生长在尼泊尔的小型紧凑喜马拉雅灌木，是一种已知的药用植物，可用于治疗喉咙痛、感冒、血液疾病、骨病、马铃薯过敏和呕吐，还可缓解肝脏疾病、头痛和背痛。本研究调查了尼泊尔丹库塔髯花杜鹃叶精油的化学成分和生物活性。通过水蒸馏法获得叶精油，并采用气相色谱-质谱联用（GC-MS）进行详细的化学分析。使用手性气相色谱法测定单萜类成分的对映体分布，这是对髯花杜鹃精油的首次手性检测。采用微量肉汤稀释试验筛选精油的抗菌活性，采用MTT法检测其对MCF-7、MDA-MB-231和5637的细胞毒性活性。从精油中总共鉴定出70种挥发性成分。主要成分是α-蒎烯（21.5%）、δ-杜松烯（13.8%）、β-蒎烯（9.5%）、柠檬烯（5.9%）、δ-紫穗槐烯（4.6%）、α-穆罗烯（4.5%）和（E）-石竹烯（3.2%），其他次要成分含量均小于3%。该精油显示出微弱的抗菌和细胞毒性活性，但无抗真菌作用。
