Poudel Darbin Kumar, Rokaya Anil, Ojha Pawan Kumar, Timsina Sujan, Satyal Rakesh, Dosoky Noura S, Satyal Prabodh, Setzer William N
Analytica Research Center, Kirtipur, Kathmandu, Nepal.
Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA.
Molecules. 2021 Aug 24;26(17):5132. doi: 10.3390/molecules26175132.
L. is grown as an ornamental plant, used as raw material for furniture, as a source of camphor, and its essential oil can be used as an important source for perfume as well as alternative medicine. A comparative investigation of essential oil compositions and antimicrobial activities of different tissues of was carried out. The essential oils were extracted by hydrodistillation with a Clevenger apparatus and their compositions were evaluated through gas chromatography-mass spectrometry (GC-MS), enantiomeric composition by chiral GC-MS, and antimicrobial properties were assayed by measuring minimum inhibitory concentrations (MICs). Different plant tissues had different extraction yields, with the leaf having the highest yield. GC-MS analysis revealed the presence of 18, 75, 87, 67, 67, and 74 compounds in leaf, branch, wood, root, leaf/branch, and leaf/branch/wood, respectively. The significance of combining tissues is to enable extraction of commercial quality essential oils without the need to separate them. The oxygenated monoterpene camphor was the major component in all tissues of except for safrole in the root. With chiral GC-MS, the enantiomeric distributions of 12, 12, 13, 14, and 14 chiral compounds in branch, wood, root, leaf/branch, and leaf/branch/wood, respectively, were determined. The variation in composition and enantiomeric distribution in the different tissues of may be attributed to the different defense requirements of these tissues. The wood essential oil showed effective antibacterial activity against with an MIC of 39.1 μg/mL. Similarly, the mixture of leaf/branch/wood essential oils displayed good antifungal activity against and while the leaf essential oil was notably active against essential oils showed variable antimicrobial activities against dermal and pulmonary-borne microbes.
L. 作为一种观赏植物种植，用作家具原材料、樟脑来源，其精油可作为香水以及替代医学的重要来源。对L. 不同组织的精油成分和抗菌活性进行了比较研究。采用克莱文杰装置通过水蒸馏法提取精油，并通过气相色谱 - 质谱联用仪（GC - MS）评估其成分，通过手性GC - MS测定对映体组成，通过测量最低抑菌浓度（MICs）测定抗菌性能。不同植物组织的提取率不同，叶片的提取率最高。GC - MS分析表明，叶片、树枝、木材、根、叶/枝和叶/枝/木中分别存在18、75、87、67、67和74种化合物。组合组织的意义在于能够提取商业品质的精油而无需将它们分离。除根部的黄樟素外，氧化单萜樟脑是L. 所有组织中的主要成分。通过手性GC - MS分别测定了树枝、木材、根、叶/枝和叶/枝/木中12、12、13、14和14种手性化合物的对映体分布。L. 不同组织中成分和对映体分布的变化可能归因于这些组织不同的防御需求。木材精油对[具体菌种]显示出有效的抗菌活性，MIC为39.1μg/mL。同样，叶/枝/木精油混合物对[具体菌种]显示出良好的抗真菌活性，而叶片精油对[具体菌种]具有显著活性。精油对皮肤和肺部微生物显示出不同的抗菌活性。
