Fraunhofer Institute for Silicate Research ISC, Translational Center Regenerative Therapies, Würzburg, Germany.
Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, Austria; COREMED - Cooperative Centre for Regenerative Medicine, Joanneum Research Forschungsgesellschaft mbH, Graz, 9 Austria.
Burns. 2019 Jun;45(4):898-904. doi: 10.1016/j.burns.2018.10.023. Epub 2018 Nov 30.
With the increase of antimicrobial resistance in recent decades, other methods of preventing and fighting infections must be considered. Burn patients, whose wound areas are often extensive, are especially prone to wound infections. The loading of bacterial nanocellulose (BNC) with antiseptics has already been successfully performed but unfortunately, the described procedure is time-consuming and thus not applicable in a clinical emergency setting. Therefore, a clinically feasible approach was established.
Sheets of BNC-based wound dressings were placed into antiseptic solutions containing PHMB (Prontosan and LAVANID 2) and were left to soak for up to two hours. At different time points, samples were analysed for their concentration of PHMB and antiseptic efficacy.
Within 30min, clinically relevant concentrations of PHMB were achieved in the BNC-based wound dressing. The 30-min PHMB uptake for Prontosan and LAVANID 2 resulted in concentrations of 0.05% and 0.019%, respectively. Samples from the PHMB loaded dressing showed a dose dependent antiseptic efficacy for Staphylococcus aureus.
This experiment showed that the loading of BNC-based wound dressings with PHMB-containing antiseptics was achieved by a simple and quick procedure. According to studies a PHMB concentration of 0.001% can already inhibits all bacterial growth, indicating that the concentrations of PHMB in the BNC-based wound dressings after 30min are higher than the minimal inhibitory concentration and the antiseptic efficacy after 120min loading analysed by an standardized bacterial disk diffusion assay was shown to be comparable to the clinically used Suprasorb X+PHMB wound dressing.
近几十年来，由于抗生素耐药性的增加，必须考虑其他预防和控制感染的方法。烧伤患者的创面往往很广泛，特别容易发生创面感染。将抗菌纳米纤维素（BNC）加载抗菌剂已经成功实施，但不幸的是，所描述的程序耗时且不适用于临床紧急情况。因此，建立了一种临床可行的方法。
将基于 BNC 的伤口敷料片放入含有 PHMB（普朗托尼和拉万迪 2）的防腐剂溶液中，浸泡长达两小时。在不同的时间点，对样品进行 PHMB 浓度和抗菌效果分析。
在 30 分钟内，基于 BNC 的伤口敷料中达到了临床相关的 PHMB 浓度。普朗托尼和拉万迪 2 的 30 分钟 PHMB 吸收量分别导致 0.05%和 0.019%的浓度。来自 PHMB 加载敷料的样品对金黄色葡萄球菌表现出剂量依赖性的抗菌效果。
该实验表明，通过简单快速的程序可以将含 PHMB 的防腐剂加载到基于 BNC 的伤口敷料上。根据研究，PHMB 浓度为 0.001%即可抑制所有细菌生长，表明 30 分钟后基于 BNC 的伤口敷料中的 PHMB 浓度高于最小抑菌浓度，并且通过标准化细菌圆盘扩散测定分析的 120 分钟加载后的抗菌效果与临床使用的 Suprasorb X+PHMB 伤口敷料相当。
