Wiegand Cornelia, Moritz Sebastian, Hessler Nadine, Kralisch Dana, Wesarg Falko, Müller Frank A, Fischer Dagmar, Hipler Uta-Christina
Department of Dermatology, University Hospital Center Jena, Erfurter Str. 35, 07740, Jena, Germany.
Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, Jena, Germany.
J Mater Sci Mater Med. 2015 Oct;26(10):245. doi: 10.1007/s10856-015-5571-7. Epub 2015 Sep 28.
Bacterial nanocellulose (BNC) is chemically identical with plant cellulose but free of byproducts like lignin, pectin, and hemicelluloses, featuring a unique reticulate network of fine fibers. BNC sheets are mostly obtained by static cultivation. Now, a Horizontal Lift Reactor may provide a cost efficient method for mass production. This is of particular interest as BNC features several properties of an ideal wound dressing although it exhibits no bactericidal activity. Therefore, BNC was functionalized with the antiseptics povidone-iodine (PI) and polihexanide (PHMB). Drug loading and release, mechanical characteristics, biocompatibility, and antimicrobial efficacy were investigated. Antiseptics release was based on diffusion and swelling according to Ritger-Peppas equation. PI-loaded BNC demonstrated a delayed release compared to PHMB due to a high molar drug mass and structural changes induced by PI insertion into BNC that also increased the compressive strength of BNC samples. Biological assays demonstrated high biocompatibility of PI-loaded BNC in human keratinocytes but a distinctly lower antimicrobial activity against Staphylococcus aureus compared to PHMB-loaded BNC. Overall, BNC loaded with PHMB demonstrated a better therapeutic window. Moreover, compressive and tensile strength were not changed by incorporation of PHMB into BNC, and solidity during loading and release could be confirmed.
细菌纳米纤维素（BNC）在化学性质上与植物纤维素相同，但不含木质素、果胶和半纤维素等副产品，具有独特的精细纤维网状网络。BNC片材大多通过静态培养获得。现在，一种卧式提升反应器可能为大规模生产提供一种经济高效的方法。这一点尤其值得关注，因为尽管BNC没有杀菌活性，但它具有理想伤口敷料的多种特性。因此，用防腐剂聚维酮碘（PI）和聚己双胍（PHMB）对BNC进行了功能化处理。研究了药物负载与释放、机械特性、生物相容性和抗菌效果。根据Ritger-Peppas方程，防腐剂的释放基于扩散和溶胀。由于高摩尔药物质量以及PI插入BNC引起的结构变化（这也增加了BNC样品的抗压强度），负载PI的BNC表现出与负载PHMB的BNC相比延迟的释放。生物学试验表明，负载PI的BNC在人角质形成细胞中具有高生物相容性，但与负载PHMB的BNC相比，对金黄色葡萄球菌的抗菌活性明显较低。总体而言，负载PHMB的BNC表现出更好的治疗窗口。此外，将PHMB掺入BNC中不会改变其抗压强度和抗拉强度，并且可以确认在负载和释放过程中的坚固性。
