Lee William R, Tobias Karen M, Bemis David A, Rohrbach Barton W
Department of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN 37996-4544, USA.
Vet Surg. 2004 Jul-Aug;33(4):404-11. doi: 10.1111/j.1532-950X.2004.04059.x.
To evaluate the in vitro efficacy of polyhexamethylene biguanide (PHMB)-impregnated gauze dressing in limiting the growth of bacteria both within and underneath the dressing.
In vitro study.
Squares of PHMB-impregnated and control gauze were placed on agar plates inoculated with 1 of 11 bacterial species, including 8 multi-resistant organisms. Growth under the gauze was assessed qualitatively after 24-hour incubation. Repeated use of sponges was used to evaluate residual inhibitory activity against Micrococcus lutea and Staphylococcus schleiferi ss. schleiferi. In a second procedure, PHMB-impregnated and control gauze squares were placed in sterile plastic wells and inoculated with 1 of 5 bacterial species, including Pseudomonas spp. and Klebsiella spp. Inhibition of bacterial growth within and underneath the dressing after 24-hour incubation was evaluated by quantifying the numbers of bacteria on the well floor and within each square.
PHMB-impregnated gauze provided greater inhibition of growth of 4/4 Gram-positive species and 2/6 Gram-negative species on inoculated plates compared with control gauze. Residual inhibitory activity of PHMB-impregnated gauze was significantly greater against M. lutea on all days and against S. schleiferi ss. schleiferi on days 1 and 4 compared with control. No bacteria were recovered from inoculated PHMB-impregnated gauze squares placed in sterile wells or from the well floor underneath. More than 9 x 10(5) colony-forming units (CFU) were recovered from inoculated control samples placed in sterile wells and more than 8.4 x 10(4) CFU were recovered from control well floors.
PHMB-impregnated gauze dressing, when placed on inoculated agar plates, reduces growth of underlying bacteria, particularly Gram-positive species. Wet-inoculated PHMB-impregnated dressing prevents growth of Gram-positive and Gram-negative bacteria both within and underneath the dressing.
PHMB-impregnated dressings may be useful for reducing contamination of underlying wounds by bacterial pathogens.
评估聚六亚甲基双胍（PHMB）浸渍纱布敷料在限制敷料内外细菌生长方面的体外效果。
体外研究。
将PHMB浸渍纱布方块和对照纱布方块放置在接种了11种细菌中的1种的琼脂平板上，其中包括8种多重耐药菌。孵育24小时后，定性评估纱布下方的细菌生长情况。使用海绵重复使用来评估对藤黄微球菌和施氏葡萄球菌施氏亚种的残留抑制活性。在第二个步骤中，将PHMB浸渍纱布方块和对照纱布方块放置在无菌塑料孔中，并接种5种细菌中的1种，包括假单胞菌属和克雷伯菌属。通过对孔底部和每个方块内的细菌数量进行定量，评估孵育24小时后敷料内外细菌生长的抑制情况。
与对照纱布相比，PHMB浸渍纱布在接种平板上对4/4种革兰氏阳性菌和2/6种革兰氏阴性菌的生长具有更强的抑制作用。PHMB浸渍纱布对藤黄微球菌在所有天数以及对施氏葡萄球菌施氏亚种在第1天和第4天的残留抑制活性均显著高于对照。在放置于无菌孔中的接种PHMB浸渍纱布方块或其下方的孔底部均未回收细菌。从放置在无菌孔中的接种对照样品中回收了超过9×10⁵菌落形成单位（CFU），从对照孔底部回收了超过8.4×10⁴CFU。
当放置在接种琼脂平板上时，PHMB浸渍纱布敷料可减少下层细菌的生长，尤其是革兰氏阳性菌。湿接种的PHMB浸渍敷料可防止革兰氏阳性菌和革兰氏阴性菌在敷料内外生长。
PHMB浸渍敷料可能有助于减少细菌病原体对下层伤口的污染。
