Knezevic Alena, Zeljezic Davor, Kopjar Nevenka, Duarte Sillas, Par Matej, Tarle Zrinka
Division of Restorative Sciences, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, US.
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Acta Stomatol Croat. 2018 Sep;52(3):203-217. doi: 10.15644/asc52/3/4.
The aim was to compare cytotoxicity/genotoxicity of pre-heated composites polymerized through CAD/CAM overlays on isolated human peripheral blood lymphocytes.
A microhybrid (Z100, 3M ESPE) and nanofilled composite (Filtek Supreme Ultra, 3M ESPE) were heated in a heating unit (Calset, AdDent Inc.) at different temperatures: 37 C, 54 C, and 68 C. A small amount of heated composite was placed in a cylindrical mold (6mm diameter; 0.65mm thick), covered with a Mylar sheet, pressed and light-cured directly and through 2 mm thick CAD/CAM ceramic-reinforced polymer (CRP)(LAVA Ultimate, 3M ESPE) or CAD/CAM lithium disilicate ceramic (LDC)(e.max, Ivoclar/Vivadent) overlay. After curing, the specimens were immediately placed in a prepared lymphocyte cell culture. Cytotoxicity was assessed using a dye exclusion method by simultaneous staining with ethidium bromide and acridine orange, aimed to determine percentages of viable, apoptotic and necrotic cells. Genotoxicity was studied using alkaline comet assay.
For Z100, the highest percentage of viable cells is recorded at T1 (93.7%) after direct light curing, followed by light curing through CRP (92.3%) and through LDC (91.7%T1,T3). For Filtek Supreme Ultra, the highest percentage of viable cells is recorded while curing through CRP (91.0% T2), followed by LDC (90% T1,T3) and direct light curing (88.7%T2).
For both tested materials, preheating the procedure at T1 and T2 may be the procedure of choice. In terms of genotoxicity, preheating at T3 may not be suggested.
比较通过计算机辅助设计/计算机辅助制造（CAD/CAM）覆盖物聚合的预热复合材料对分离的人外周血淋巴细胞的细胞毒性/遗传毒性。
将一种微混合复合材料（Z100，3M ESPE公司）和一种纳米填充复合材料（Filtek Supreme Ultra，3M ESPE公司）在加热单元（Calset，AdDent公司）中于不同温度下加热：37℃、54℃和68℃。将少量加热后的复合材料放入圆柱形模具（直径6mm；厚0.65mm）中，覆盖聚酯薄膜片，直接加压并光固化，以及通过2mm厚的CAD/CAM陶瓷增强聚合物（CRP）（LAVA Ultimate，�M ESPE公司）或CAD/CAM二硅酸锂陶瓷（LDC）（e.max，义获嘉伟瓦登特公司）覆盖物进行光固化。固化后，将标本立即放入准备好的淋巴细胞细胞培养物中。使用溴化乙锭和吖啶橙同时染色的染料排除法评估细胞毒性，旨在确定活细胞、凋亡细胞和坏死细胞百分比。使用碱性彗星试验研究遗传毒性。
对于Z100，直接光固化后在T1时记录到的活细胞百分比最高（93.7%），其次是通过CRP光固化（92.3%）和通过LDC光固化（91.7%，T1、T3）。对于Filtek Supreme Ultra，通过CRP固化时记录到的活细胞百分比最高（91.0%，T2），其次是LDC（90%，T1、T3）和直接光固化（88.7%，T2）。
对于两种测试材料，在T1和T2进行预热程序可能是首选程序。在遗传毒性方面，不建议在T3进行预热。
