Karan Tunay
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Gaziosmanpasa University, 60240 Tokat, Turkey.
Cell Mol Biol (Noisy-le-grand). 2018 Nov 30;64(14):1-7.
Medicinal and aromatic plants play a significant role in drug discovery. Lavandula stoechas L. has been used as folk medicine to treat various diseases. The aim of this work is to investigate the phytochemisty of Lavandula stoechas with biological activities. An aerial part of the plant was extracted with methanol. Another sample of plant was boiled in water then aqueous part was extracted with ethyl acetate. Identification and quantification of phenolic compounds, organic acids and flavonoids in methanol extract were carried out by High Performance Liquid Chromatography/Time of Flight/Mass Spectrometry, HPLC-TOF/MS. Rosmarinic acid was found as a chief compound (80.9%). The essential oil was generated by steam distillation and identified by GC-MS. The main constituents were camphor (48.1%) and fenchone (30.5%). The essential oil exhibited good insecticidal activity on Sitophilus granarius and Sitophilus oryzae pests as 43.3% and 62.9% mortality, respectively. However, the methanol extract has only insecticidal activity against S. granaries (50.0%). The antioxidant activities were investigated using assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging, reducing power (FRAP), and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS•+) radical scavenging on both extracts. In addition, total phenolic contents were determined. Total phenolic content of EtOAc extract was found as 2.18 g GAE (Gallic acid equivalent) phenolic compounds/kg plant. Whereas, total phenolic of methanol was 3.33 g GAE/kg plant. EtOAc extract revealed the considerable DPPH• scavenging, ABTS•+ scavenging and reducing power activities with the values of 28.71 (IC50, µg/ml), 8.72 (IC50, µg/ml) and 6.99 (µmol trolox equivalent/mg extract) respectively.
药用植物和芳香植物在药物发现中发挥着重要作用。意大利薰衣草已被用作民间药物来治疗各种疾病。这项工作的目的是研究意大利薰衣草的植物化学及其生物活性。用甲醇提取该植物的地上部分。将另一部分植物样品在水中煮沸，然后将水相部分用乙酸乙酯萃取。采用高效液相色谱/飞行时间/质谱联用技术（HPLC-TOF/MS）对甲醇提取物中的酚类化合物、有机酸和黄酮类化合物进行鉴定和定量。发现迷迭香酸是主要成分（80.9%）。通过水蒸气蒸馏法提取精油，并采用气相色谱-质谱联用技术（GC-MS）进行鉴定。主要成分是樟脑（48.1%）和小茴香酮（30.5%）。该精油对谷象和米象害虫表现出良好的杀虫活性，死亡率分别为43.3%和62.9%。然而，甲醇提取物仅对谷象具有杀虫活性（50.0%）。使用1,1-二苯基-2-苦基肼（DPPH•）自由基清除、还原力（FRAP）和2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS•+）自由基清除试验对两种提取物的抗氧化活性进行了研究。此外，还测定了总酚含量。乙酸乙酯提取物的总酚含量为2.18 g GAE（没食子酸当量）酚类化合物/千克植物。而甲醇提取物的总酚含量为3.33 g GAE/千克植物。乙酸乙酯提取物表现出显著的DPPH•清除、ABTS•+清除和还原力活性，其值分别为28.71（IC50，μg/ml）、8.72（IC50，μg/ml）和6.99（μmol Trolox当量/毫克提取物）。
