Kim Hyojin, Chung Mi Sook
Department of Food and Nutrition, Duksung Women's University, Seoul 01369, Republic of Korea.
Evid Based Complement Alternat Med. 2018 Nov 1;2018:2606583. doi: 10.1155/2018/2606583. eCollection 2018.
Antiviral activities of (MA) juice and seed were examined using time-of-addition plaque assays against influenza viruses, A/Brisbane/59/2007 (H1N1) (BR59), pandemic A/Korea/01/2009(H1N1) (KR01), A/Brisbane/10/2007(H3N2) (BR10), and B/Florida/4/2006 (FL04). MA juice (MAJ) showed much higher antiviral activity than MA seed (MAS). In the pre- and cotreatment of virus, MAJ showed antiviral effects against BR59, KR01, and FL04 in a dose-dependent manner. In particular, MAJ at 4% concentration exhibited 1.3 log inhibition in the pre- and cotreatment of the virus against FL04, a type B virus. However, little or weak inhibition was observed in the posttreatment of MAJ. GSH levels in the virus-infected cells were also examined. The decreased levels by the viral infection were restored significantly by the addition of MAJ. MAJ also exhibited significant DPPH radical scavenging and ferric ion-reducing activities in a dose-dependent manner. Cyanidin-3-rutinoside, the most abundant polyphenol compound of MAJ identified by LC-MS in this study, showed weak inhibitory effects against FL04 in the pretreatment, whereas gallic acid, a minor compound of MAJ, revealed significant antiviral effect. These results suggest that MAJ can be developed as a novel plant-derived antiviral against influenza viruses.
采用加样时间空斑试验检测了（MA）果汁和种子对流感病毒A/布里斯班/59/2007（H1N1）（BR59）、大流行A/韩国/01/2009（H1N1）（KR01）、A/布里斯班/10/2007（H3N2）（BR10）和B/佛罗里达/4/2006（FL04）的抗病毒活性。MA果汁（MAJ）显示出比MA种子（MAS）高得多的抗病毒活性。在病毒的预处理和共处理中，MAJ对BR59、KR01和FL04呈现出剂量依赖性的抗病毒作用。特别是，4%浓度的MAJ在病毒对B型病毒FL04的预处理和共处理中表现出1.3个对数级的抑制作用。然而，MAJ在病毒感染后处理中观察到的抑制作用很小或较弱。还检测了病毒感染细胞中的谷胱甘肽（GSH）水平。病毒感染导致的GSH水平降低通过添加MAJ得到了显著恢复。MAJ还以剂量依赖性方式表现出显著的1,1-二苯基-2-苦基肼（DPPH）自由基清除和铁离子还原活性。本研究通过液相色谱-质谱联用（LC-MS）鉴定出MAJ中最丰富的多酚化合物矢车菊素-3-芸香糖苷在预处理中对FL04显示出较弱的抑制作用，而MAJ中的次要化合物没食子酸则显示出显著的抗病毒作用。这些结果表明，MAJ可被开发成为一种新型的植物源抗流感病毒药物。
