Samart Nuttaporn, Arhouma Zeyad, Kumar Santosh, Murakami Heide A, Crick Dean C, Crans Debbie C
Department of Chemistry, Colorado State University, Fort Collins, CO, United States.
Department of Chemistry, Rajabhat Rajanagarindra University, Chachoengsao, Thailand.
Front Chem. 2018 Nov 20;6:519. doi: 10.3389/fchem.2018.00519. eCollection 2018.
V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of () and () was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V ) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.
钒核磁共振光谱法用于通过物种分析记录，一种氧金属酸盐比其他氧钒酸盐对两种分枝杆菌菌株的生长抑制作用更强，从而证明其与细胞相互作用具有选择性。从历史上看，氧金属酸盐在生物学和医学研究中有许多应用，包括核糖体X射线晶体学中相位问题的研究。研究了不同钒酸盐对（）和（）生长的影响，发现物种形成对于观察到的生长抑制至关重要。具体而言，发现大的橙色十钒酸钠（V）阴离子比由偏钒酸钠制备的无色氧钒酸盐对两种分枝杆菌的生长抑制作用更强。使用钒核磁共振光谱法和物种形成计算监测生长培养基中的钒（V）物种形成以及生长条件下物种之间的转化。这项工作中提出的发现对于考虑多金属氧酸盐在生物学和医学研究中的许多应用尤为重要，例如核糖体X射线晶体学中相位问题的研究。这项工作中提出的发现研究了氧金属酸盐与其他生物系统的相互作用。
