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氘对人培养脂肪干细胞生物学特性影响的研究

Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells.

作者信息

Zlatska Alona, Gordiienko Inna, Vasyliev Roman, Zubov Dmitriy, Gubar Olga, Rodnichenko Anzhela, Syroeshkin Anton, Zlatskiy Igor

机构信息

State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.

Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine.

出版信息

ScientificWorldJournal. 2018 Nov 4;2018:5454367. doi: 10.1155/2018/5454367. eCollection 2018.

DOI:10.1155/2018/5454367
PMID:30519147
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6241234/
Abstract

In current study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.

摘要

在当前研究中，我们已经表明生长培养基中氘含量对人培养脂肪来源干细胞（ADSC）增殖速率的影响。当在含氘生长培养基中培养时，ADSC也表现出形态变化：细胞培养物未达到汇合，但获得了具有明显应力纤维的多边形形态。在高氘浓度下，ADSC群体倍增时间增加，这表明细胞周期延迟和细胞增殖速率降低。含氘和贫氘生长培养基分别表现出急性和慢性细胞毒性。在含氘培养基中观察到最小迁移能力，而在氘含量接近天然的培养基中观察到最高迁移活性。在含氘生长培养基中的细胞在培养三天后代谢活性降低。相比之下，在贫氘培养基中ADSC的代谢活性增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/f9fe3bfac8f0/TSWJ2018-5454367.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/1524aae93a96/TSWJ2018-5454367.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/7297ab368bae/TSWJ2018-5454367.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/341659d305b2/TSWJ2018-5454367.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/9e2c1a40fa45/TSWJ2018-5454367.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/e335b03d5a38/TSWJ2018-5454367.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/f9fe3bfac8f0/TSWJ2018-5454367.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/1524aae93a96/TSWJ2018-5454367.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/7297ab368bae/TSWJ2018-5454367.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/341659d305b2/TSWJ2018-5454367.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/9e2c1a40fa45/TSWJ2018-5454367.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/e335b03d5a38/TSWJ2018-5454367.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c3a/6241234/f9fe3bfac8f0/TSWJ2018-5454367.006.jpg

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