Deineka Volodymyr, Sulaieva Oksana, Pernakov Mykola, Korniienko Viktoriia, Husak Yevheniia, Yanovska Anna, Yusupova Aziza, Tkachenko Yuliia, Kalinkevich Oksana, Zlatska Alena, Pogorielov Maksym
Medical Institute, Sumy State University, 40007 Sumy, Ukraine.
Medical Laboratory CSD, 03148 Kyiv, Ukraine.
Biomedicines. 2021 May 21;9(6):588. doi: 10.3390/biomedicines9060588.
The application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEO-copolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.
壳聚糖（Ch）作为一种具有止血特性和高生物相容性的有前景的生物聚合物，其应用受到限制，因为其降解时间延长，这反过来又减缓了修复过程。在本研究中，我们旨在开发新技术以缩短用于止血应用的基于壳聚糖材料的生物降解时间。本研究旨在评估通过静电纺丝技术制备的壳聚糖-聚环氧乙烷共聚物（Ch-PEO）的生物相容性、止血和组织再生性能。壳聚糖静电纺丝膜（ChEsM）由壳聚糖和聚环氧乙烷（PEO）粉末制成，用于制备具有足够止血参数的富含高孔隙率材料。对ChEsM的结构、孔隙率、密度、抗菌性能、体外降解和生物相容性进行了评估，并与传统的壳聚糖海绵（ChSp）进行了比较。此外，使用大鼠肝出血模型在体内检测了两种材料的止血和生物活性性能。对左肝叶进行穿透性穿刺活检以模拟不可压缩不规则伤口的出血。将形状合适的ChSp或ChEsM应用于组织损伤处。静电纺丝使我们能够生产出具有与ChSp相似降解和膨胀特性的高孔隙率膜。两种材料在体外均表现出高生物相容性和止血效果。然而，与ChSp相比，ChEsM的抗菌性能较差。体内研究证实了ChEsM具有卓越的生物相容性和足够的止血性能，与宿主细胞和组织紧密相互作用。体内模型显示ChEsM具有更高的生物降解率和更先进的肝脏修复效果。
