Department of Food Science, University of Wisconsin-Madison, Madison, WI, 53706, USA.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Mol Nutr Food Res. 2019 Mar;63(5):e1800985. doi: 10.1002/mnfr.201800985. Epub 2018 Dec 13.
Increased fruit consumption is associated with reduced risk of colitis. It has been investigated whether the anti-colitic effects of the polyphenol-rich aronia berry (Aronia mitschurinii 'Viking') are mediated through Th17 and Treg.
Colitis is induced in recombinase activating gene-1 deficient mice injected with syngeneic CD4 CD62L naïve T cells. Mice consume either 4.5% w/w aronia-berry-supplemented or a control diet concurrent with T cell transfer. The extent of colitis and immunocyte populations are evaluated at weeks 3 to 7 after transfer. Aronia consumption prevents colitic wasting and reduces colon weight/length ratios relative to the control diet at weeks 5 and 7. Compared to the control diet, aronia feeding increases Treg in mesenteric lymph node at all colitis stages. Treg and regulatory Th17 subpopulations (IL-17A IL-10 and IL-17A IL-22 ) are increased in lamina propria and spleen at week 5 in aronia-fed mice. Aronia feeding also decreases total CD4 cells but increases colonic Tregs. The ability of aronia to modulate colonic cytokines is associated with functional T cell IL-10 and increased diversity of microbiota.
Aronia berry consumption inhibits adoptive transfer colitis by increasing Treg and regulatory Th17 cells. Dietary modulation of T cells is dynamic and precedes colitic wasting.
增加水果摄入与降低结肠炎风险有关。人们研究了富含多酚的黑穗醋栗（Aronia mitschurinii 'Viking'）的抗结肠炎作用是否通过 Th17 和 Treg 介导。
在注射同基因 CD4 CD62L 幼稚 T 细胞的重组激活基因-1 缺陷型小鼠中诱导结肠炎。小鼠在 T 细胞转移的同时，分别摄入 4.5%w/w 黑穗醋栗补充剂或对照饮食。在转移后 3 至 7 周评估结肠炎的严重程度和免疫细胞群。与对照饮食相比，黑穗醋栗消耗可预防结肠炎引起的消瘦，并降低第 5 周和第 7 周的结肠重量/长度比。与对照饮食相比，黑穗醋栗喂养在所有结肠炎阶段均增加肠系膜淋巴结中的 Treg。在黑穗醋栗喂养的小鼠中，第 5 周时，调节性 Th17 亚群（IL-17A IL-10 和 IL-17A IL-22）在固有层和脾脏中的 Treg 和调节性 Th17 亚群增加。黑穗醋栗喂养还减少了总 CD4 细胞，但增加了结肠 Treg。黑穗醋栗调节结肠细胞因子的能力与功能性 T 细胞 IL-10 有关，并增加了微生物群的多样性。
黑穗醋栗消耗通过增加 Treg 和调节性 Th17 细胞来抑制 adoptive transfer 结肠炎。T 细胞的饮食调节是动态的，并且先于结肠炎引起的消瘦。
