Fang Xia, Zhang Tingran, Yang Mengliu, Li Ling, Zhang Cheng, Hu Wenjing, Fan Xiaoyun, Liu Hua, Zhu Zhiming, Liu Dongfang, Zheng Hongting, Gu Harvest F, Liu Rui, Yang Gangyi
Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University and Chongqing Clinical Research Center for Geriatrics, Chongqing, China.
College of Physical Education, Southwest University, Key Laboratory of Physical Evaluation and Sports Performance Monitoring of the State Administration of Sports, Chongqing, China.
Cell Physiol Biochem. 2018;51(5):2041-2051. doi: 10.1159/000495823. Epub 2018 Dec 6.
BACKGROUND/AIMS: Alarin has been reported to be related with increased food intake and body weight. The relationship of circulating Alarin with insulin resistance or metabolic syndrome (MetS), however, is unknown. This study aimed to investigate the physiological role of Alarin and its association with MetS in humans.
Newly diagnosed MetS patients (n=237) and age-matched healthy subjects (n=192) were recruited for this study. Oral glucose tolerance test, treadmill exercise, lipid infusions and euglycemic-hyperinsulinemic clamp (EHCs) were performed. Circulating Alarin and TNFα levels were measured by ELISA.
Circulating Alarin levels were significantly higher in MetS patients compared with healthy subjects (0.46 ± 0.22 vs. 0.41 ± 0.14 µg/L, P < 0.01). In all studied subjects, circulating Alarin levels were positively correlated with WC, blood pressure, FBG, triglyceride, HbA1c, HOMA-IR, AUCglucose, and TNFα (P < 0.05 or P < 0.01). Multivariate logistic regression analyses revealed that circulating Alarin levels were correlated with MetS and insulin resistance. There was no significant change of circulating Alarin levels in the subjects with treadmill exercise for 45 min. In healthy individuals, however, glucose challenge, acute hyperglycemia and lipid infusions resulted in increased circulating Alarin levels, while acute hyperinsulinaemia transiently decreased circulating Alarin levels.
The present study provides the evidence that circulating Alarin levels are associated with MetS and insulin resistance.
背景/目的：据报道，阿拉瑞林与食物摄入量增加和体重增加有关。然而，循环中的阿拉瑞林与胰岛素抵抗或代谢综合征（MetS）之间的关系尚不清楚。本研究旨在探讨阿拉瑞林在人体中的生理作用及其与MetS的关联。
本研究招募了新诊断的MetS患者（n = 237）和年龄匹配的健康受试者（n = 192）。进行了口服葡萄糖耐量试验、跑步机运动、脂质输注和正常血糖高胰岛素钳夹试验（EHCs）。通过酶联免疫吸附测定法测量循环中的阿拉瑞林和肿瘤坏死因子α水平。
与健康受试者相比，MetS患者循环中的阿拉瑞林水平显著更高（0.46±0.22对0.41±0.14μg/L，P < 0.01）。在所有研究对象中，循环中的阿拉瑞林水平与腰围、血压、空腹血糖、甘油三酯、糖化血红蛋白、稳态模型评估的胰岛素抵抗指数、葡萄糖曲线下面积和肿瘤坏死因子α呈正相关（P < 0.05或P < 0.01）。多因素逻辑回归分析显示，循环中的阿拉瑞林水平与MetS和胰岛素抵抗相关。进行45分钟跑步机运动的受试者循环中的阿拉瑞林水平没有显著变化。然而，在健康个体中，葡萄糖激发试验、急性高血糖和脂质输注导致循环中的阿拉瑞林水平升高，而急性高胰岛素血症则使循环中的阿拉瑞林水平短暂降低。
本研究提供了证据表明循环中的阿拉瑞林水平与MetS和胰岛素抵抗相关。
