XRE-DUF397蛋白对Scr1和Scr2作为……中抗生素产生的强正调控因子。

The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in .

作者信息

Santamaría Ramón I, Sevillano Laura, Martín Jesús, Genilloud Olga, González Ignacio, Díaz Margarita

机构信息

Instituto de Biología Funcional y Genómica, Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Científicas, Universidad de Salamanca, Salamanca, Spain.

Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Granada, Spain.

出版信息

Front Microbiol. 2018 Nov 16;9:2791. doi: 10.3389/fmicb.2018.02791. eCollection 2018.

The xenobiotic response element (XRE) transcription factors belong to a regulator family frequently found in that are often followed by small proteins with a DUF397 domain. In fact, the pair XRE-DUF397 has been proposed to comprise toxin-antitoxin (TA) type II systems. In this work, we demonstrate that one of these putative TA-systems, encoded by the genes and of , and denominated Scr1/Scr2 (which stands for . ), does not behave as a toxin-antitoxin system under the conditions used as was originally expected. Instead the pair Scr1/Scr2 acts as a strong positive regulator of endogenous antibiotic production in . The analysis of the 19 strains tested determined that overexpression of the pair Scr1/Scr2 drastically induces the production of antibiotics not only in , but also in , and sp. CA-240608. Our work also shows that Scr1 needs Scr2 to exert positive regulation on antibiotic production.

异生素反应元件（XRE）转录因子属于一个在……中经常发现的调节因子家族，其后面常常跟着具有DUF397结构域的小蛋白。事实上，有人提出XRE - DUF397这一对组成了II型毒素 - 抗毒素（TA）系统。在这项工作中，我们证明了其中一个由……的基因……编码的假定TA系统，命名为Scr1/Scr2（代表……），在最初预期的使用条件下并不表现为毒素 - 抗毒素系统。相反，Scr1/Scr2这一对在……中作为内源性抗生素产生的强正调节因子起作用。对所测试的19株……菌株的分析确定，Scr1/Scr2这一对的过表达不仅在……中，而且在……、……和……sp. CA - 240608中都能显著诱导抗生素的产生。我们的工作还表明，Scr1需要Scr2来对抗生素产生发挥正调节作用。