Department of Plant Protection, College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Poeple's Republic of China.
Department of Entomology, China Agricultural University, Beijing, People's Republic of China.
Pest Manag Sci. 2019 Jun;75(6):1726-1733. doi: 10.1002/ps.5293. Epub 2019 Jan 8.
Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing.
In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance-associated gene (MRA), GATA-binding gene (GAT) and takeout-like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid.
The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry.
杀虫剂作为毒素、消化抑制剂和驱虫剂，影响昆虫中许多基因的表达。为了评估与棉蚜（Schizaphis graminum （Rondani））中吡虫啉解毒或调节相关的关键基因，使用 Illumina 测序对转录组和数字基因表达（DGE）谱进行了分析。
通过测序共获得 48763494 条清洁读数。表达谱分析表明，吡虫啉处理组和对照组之间有 2782 个基因表达不同。与对照组相比，暴露于吡虫啉后，1846 个基因的表达水平上调，936 个基因下调。前 20 个高表达基因的表达模式表明，它们可能参与了吡虫啉的解毒。多药耐药相关基因（MRA）、GATA 结合基因（GAT）和取出样前体基因（TLP）的沉默导致对吡虫啉的敏感性增加。
棉蚜中差异表达的基因可能在对吡虫啉的反应中具有潜在的调节或解毒作用。这些结果有助于理解棉蚜适应吡虫啉的分子机制。© 2018 化学工业协会。
