Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
College of Plant Protection, Hunan Agricultural University, Changsha 410128, PR China.
Pestic Biochem Physiol. 2019 Jan;153:136-143. doi: 10.1016/j.pestbp.2018.11.014. Epub 2018 Nov 20.
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), a globally invasive species complex that causes serious damage to field crops, has developed resistance to imidacloprid and many other pesticides. Insect detoxify to pesticides may partially depend on ABC transporters, which contribute to the detoxification of xenobiotics. To determine whether genes in the ABCG subfamily are involved in imidacloprid detoxification in B. tabaci Q, we cloned four ABCG subfamily genes based on the published MED/Q genome and on our previous study of the transcriptional response of ABC transporters in B. tabaci Q adults to imidacloprid. As indicated by the quantification of mRNA levels after a 6-h exposure, the expression level of ABCG3 was 3.3-fold higher in B. tabaci Q adults exposed to 100 μg/mL imidacloprid rather than to the buffer control. The expression level of ABCG3 was higher in females than in males but did not significantly differ among eggs or nymphal stages and did not significantly differ among head, thorax, and abdomen tissues of adults. Knockdown of ABCG3 via RNA interference significantly increased the mortality of imidacloprid-treated laboratory and field-collected adults of B. tabaci Q. These results indicate that the ABCG3 gene may be involved in imidacloprid detoxification by B. tabaci Q.
烟粉虱（Bemisia tabaci）（半翅目：粉虱科）是一种全球性的入侵物种复合体，对大田作物造成严重损害，已经对吡虫啉和许多其他杀虫剂产生了抗性。昆虫对杀虫剂的解毒作用可能部分取决于 ABC 转运蛋白，ABC 转运蛋白有助于外来化合物的解毒。为了确定 ABCG 亚家族的基因是否参与烟粉虱 Q 对吡虫啉的解毒作用，我们根据已发表的 MED/Q 基因组和我们之前对烟粉虱 Q 成虫中 ABC 转运蛋白对吡虫啉转录反应的研究，克隆了四个 ABCG 亚家族基因。根据暴露于 100μg/ml 吡虫啉后 6 小时的 mRNA 水平定量，暴露于吡虫啉而非缓冲液对照的烟粉虱 Q 成虫中 ABCG3 的表达水平增加了 3.3 倍。ABC3 在雌性中的表达水平高于雄性，但在卵或若虫阶段之间没有显著差异，在成虫的头部、胸部和腹部组织之间也没有显著差异。通过 RNA 干扰敲低 ABCG3 显著增加了实验室和田间采集的烟粉虱 Q 成虫对吡虫啉的死亡率。这些结果表明，ABCG3 基因可能参与了烟粉虱 Q 对吡虫啉的解毒作用。
