1 Gastroenterology Unit, IRCCS Casa Sollievo della Sofferenza Hospital, viale dei Cappuccini n. 1, 71013 San Giovanni Rotondo (FG), Italy.
2 Laboratories of General Pathology and Immunology, Department of Medicine and Surgery, School of Medicine, University of Insubria, via O. Rossi 9 - Padiglione Biffi, 21100 Varese, Italy.
Benef Microbes. 2019 Feb 8;10(1):69-75. doi: 10.3920/BM2018.0060. Epub 2018 Dec 10.
The incidence of asthma and allergic diseases of the airways is constantly increasing, both in the industrialised and developing countries, due to harmful and excessive quantities of air pollution. Although some studies have shown an effect of dietary supplementation of specific nutrients (especially with anti-oxidant and anti-inflammatory properties) in reducing airways inflammatory response, the results are not yet conclusive and the science is still at its infancy. Our hypothesis is that combining such nutrients could provide more benefits than using them alone. The aim of the research project proposed here is to investigate whether specific combinations of nutrients (docosahexanoic acid, vitamin C and E, and Bifidobacterium lactis strain BB-12®, included in an engineered diet) can act synergistically to reduce inflammation given by high level of air pollution. Beside the role of docosahexanoic acid, vitamins C and E on airways inflammatory disease, no study examined the effect of the supplementation of this probiotic strain in pathological conditions caused by air pollution so far. Herein we used a well-established in vivo model for the study of pollution effects, which consists in female BALB/c mice receiving by pharyngeal aspiration either a sham or a particulate matter with diameter <2.5 μm (PM 2.5) containing aerosol. Before treatment, mice were fed either a chow or a supplemented diet. By performing histological analyses and gene expression profiles on lung sections and serum measurement of the cytokine interleukin 10, we found that a specific combination of all the aforementioned nutrients rather than nutrients alone had a synergistic protective effect against PM2.5-induced inflammation. In conclusion, our study support that a supplemental nutritional intervention based on a combination of the probiotic B. lactis BB-12, the anti-oxidant vitamin C and E, and the anti-inflammatory docosahexanoic acid represents a rational option for alleviating air pollution-related lung inflammation.
哮喘和气道过敏性疾病的发病率不断上升，无论是在工业化国家还是发展中国家，这都归因于有害和过量的空气污染。虽然一些研究表明，膳食补充特定营养素（特别是具有抗氧化和抗炎特性的营养素）可减少气道炎症反应，但结果尚不确定，且相关科学仍处于起步阶段。我们假设将这些营养素结合使用可能会比单独使用效果更好。这里提出的研究项目旨在研究特定营养素（二十二碳六烯酸、维生素 C 和 E，以及双歧杆菌乳双歧杆菌 BB-12 ® ，包含在一种工程化饮食中）的组合是否可以协同作用，以减少高水平空气污染引起的炎症。除了二十二碳六烯酸、维生素 C 和 E 对气道炎症性疾病的作用外，目前尚无研究检查过这种益生菌菌株在空气污染引起的病理条件下的补充效果。在这里，我们使用了一种已建立的体内模型来研究污染的影响，该模型由通过咽吸入接受模拟或包含直径<2.5μm 的颗粒物（PM 2.5 ）的气溶胶的雌性 BALB/c 小鼠组成。在治疗前，小鼠喂食标准饮食或补充饮食。通过对肺组织切片进行组织学分析和基因表达谱分析以及对血清细胞因子白细胞介素 10 的测量，我们发现，所有上述营养素的特定组合而不是单独的营养素对 PM 2.5 诱导的炎症具有协同保护作用。总之，我们的研究支持基于益生菌乳双歧杆菌 BB-12、抗氧化维生素 C 和 E 以及抗炎性二十二碳六烯酸的组合的补充性营养干预措施是缓解与空气污染相关的肺部炎症的合理选择。
