Koprowska I, Zipfel S A, Himes T R, Herlyn M
Cancer Res. 1985 Nov;45(11 Pt 2):5964-8.
The binding characteristics of monoclonal antibodies produced against a variety of human tumor cells were studied on cervical carcinoma cell lines and on exfoliated cells of cervical smears. The latter included normal epithelial cells, cells derived from cervical intraepithelial neoplasia, and cells from squamous cell carcinoma. Monoclonal antibodies that bound in immunoperoxidase assays to ethanol-fixed smears of cultured human tumor cells but not to normal cervical smears were screened on cervical smears containing malignant cells. Of the six antibodies selected for detailed studies, two each had been produced against bladder carcinoma and melanoma and one each against cervical and gastric carcinoma. Antibody 99-57 stained malignant cells from invasive carcinoma but not normal cervical cells. In cells from intraepithelial neoplasia, staining intensity was highest in severely dysplastic cells. Thus monoclonal antibodies are potentially useful in the detection of malignant cervical cells within a large number of nonmalignant cells, in conjunction with other diagnostic procedures.
我们研究了针对多种人类肿瘤细胞产生的单克隆抗体在宫颈癌细胞系和宫颈涂片脱落细胞上的结合特性。后者包括正常上皮细胞、宫颈上皮内瘤变衍生细胞以及鳞状细胞癌细胞。在含有恶性细胞的宫颈涂片上筛选出在免疫过氧化物酶试验中能与培养的人类肿瘤细胞的乙醇固定涂片结合但不与正常宫颈涂片结合的单克隆抗体。在挑选出进行详细研究的六种抗体中，两种是针对膀胱癌和黑色素瘤产生的，另外两种分别针对宫颈癌和胃癌。抗体99 - 57可对浸润性癌的恶性细胞染色，但不能对正常宫颈细胞染色。在上皮内瘤变的细胞中，重度发育异常细胞的染色强度最高。因此，单克隆抗体与其他诊断程序相结合，在大量非恶性细胞中检测恶性宫颈细胞方面可能具有潜在用途。
