College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; Henan Engineering Research Center of Food Material, Henan University of Science and Technology, Luoyang 471023, China.
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; National Demonstration Center for Experimental Food Processing and Safety Education, Henan University of Science and Technology, Luoyang 471023, China.
Int J Biol Macromol. 2019 Mar 1;124:955-962. doi: 10.1016/j.ijbiomac.2018.12.035. Epub 2018 Dec 4.
Paeonia suffruticosa Andr. has a long history of cultivation in China and its flower possesses high nutritional and medicinal value. Characterization and macrophage immunomodulatory activity of the two polysaccharides from the flowers of Paeonia suffruticosa Andr., PSAP-1 and PSAP-2, purified by anion exchange chromatography followed by size exclusion chromatography, were investigated in the present study. Results showed that PSAP-1, with a molecular weight of 155 kDa, was mainly composed of glucuronic acid, glucose, arabinose and galactose with molar percentages of 0.83, 11.53, 18.98 and 68.96% respectively, and PSAP-2, with a molecular weight of 210 kDa, consisted of rhamnose, galacturonic acid, glucose, arabinose and galactose with molar percentages of 9.13, 8.35, 12.20, 14.75 and 55.57% respectively. Immunostimulatory activity evaluation in vitro revealed that the PSAP-1 and PSAP-2 could significantly stimulate the proliferation of Raw264.7 cells in dose-dependent manner and further activate Raw264.7 cells by releasing immunoactive molecules such as nitric oxide, tumor necrosis factor (TNF)-α and interleukin (IL)-6. In addition, PSAP-2 had higher immunomodulatory activity than PSAP-1. The above results suggested that both PSAP-1 and PSAP-2 had potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent in medicine or functional food fields.
牡丹皮是中国传统的药用植物，具有悠久的栽培历史。其花瓣具有很高的营养价值和药用价值。本研究通过阴离子交换色谱和凝胶渗透色谱对从牡丹皮中分离得到的两种多糖 PSAP-1 和 PSAP-2 进行了表征，并研究了其对巨噬细胞的免疫调节活性。结果表明，PSAP-1 的分子量为 155 kDa，主要由葡萄糖醛酸、葡萄糖、阿拉伯糖和半乳糖组成，摩尔百分比分别为 0.83、11.53、18.98 和 68.96%；PSAP-2 的分子量为 210 kDa，由鼠李糖、半乳糖醛酸、葡萄糖、阿拉伯糖和半乳糖组成，摩尔百分比分别为 9.13、8.35、12.20、14.75 和 55.57%。体外免疫刺激活性评价结果表明，PSAP-1 和 PSAP-2 能够以剂量依赖的方式显著刺激 Raw264.7 细胞的增殖，并通过释放一氧化氮、肿瘤坏死因子-α和白细胞介素-6 等免疫活性分子进一步激活 Raw264.7 细胞。此外，PSAP-2 比 PSAP-1 具有更高的免疫调节活性。上述结果表明，PSAP-1 和 PSAP-2 均具有较强的免疫刺激活性，可作为一种潜在的天然免疫调节剂，应用于医学或功能性食品领域。
