College of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong Province 510640, China.
College of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong Province 510640, China.
Carbohydr Polym. 2020 Oct 15;246:116551. doi: 10.1016/j.carbpol.2020.116551. Epub 2020 Jun 15.
Chinese water chestnut peels are a kind of vegetable processing waste containing many active components such as polysaccharides, the structure of which remains unknown. To elucidate the structure of polysaccharides from Chinese water chestnut peels, two polysaccharides named WVP-1 and WVP-2 were isolated. WVP-1 (3.16 kDa) consisted of mannose (1.75 %), glucose (84.69 %), galactose (6.32 %), and arabinose (7.24 %), while WVP-2 (56.97 kDa) was composed of mannose (3.18 %), rhamnose (1.52 %), glucuronic acid (1.42 %), galacturonic acid (4.83 %), glucose (11.51 %), galactose (36.02 %), and arabinose (41.53 %). Linkage and NMR data indicated that WVP-1 was composed mainly of →4)-α-d-Glcp(1→ and a certain proportion of →3)-β-d-Glcp-(1→, including linear and branched polysaccharides simultaneously. WVP-2 was a pectin-like polysaccharide with →4)-α-d-GalpA6Me-(1→ units and the branch points of →3,4)-α-l-Arap-(1→, →3,6)-β-d-Galp-(1→. WVP-2 exhibited stronger potential antioxidant and immunomodulatory activities than WVP-1 in vitro. These results provide a foundation for the further study of polysaccharides from Chinese water chestnut peels.
菱角皮是一种蔬菜加工废弃物，含有多种活性成分，如多糖，其结构尚不清楚。为阐明菱角皮多糖的结构，从菱角皮中分离得到两种多糖，命名为 WVP-1 和 WVP-2。WVP-1（3.16 kDa）由甘露糖（1.75%）、葡萄糖（84.69%）、半乳糖（6.32%）和阿拉伯糖（7.24%）组成，而 WVP-2（56.97 kDa）由甘露糖（3.18%）、鼠李糖（1.52%）、葡萄糖醛酸（1.42%）、半乳糖醛酸（4.83%）、葡萄糖（11.51%）、半乳糖（36.02%）和阿拉伯糖（41.53%）组成。糖苷键和 NMR 数据表明，WVP-1 主要由→4）-α-d-Glcp（1→和一定比例的→3）-β-d-Glcp-（1→组成，同时含有线性和支链多糖。WVP-2 是一种类似于果胶的多糖，含有→4）-α-d-GalpA6Me-（1→单元和分支点→3,4）-α-l-Arap-（1→、→3,6）-β-d-Galp-（1→。WVP-2 在体外表现出比 WVP-1 更强的潜在抗氧化和免疫调节活性。这些结果为进一步研究菱角皮多糖提供了基础。
