College of Marine Life Science, Ocean University of, China.
CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189, Songling Road, Qingdao 266101, China.
Int J Biol Macromol. 2019 Mar 15;125:78-86. doi: 10.1016/j.ijbiomac.2018.12.058. Epub 2018 Dec 5.
Cell therapy with bone marrow-derived mesenchymal stem cells (BMSCs) is a potential method for many disease treatments, including keloid. In the present study, an Arg-Gly-Asp (RGD) modified hydroxybutyl chitosan (HBC) hydrogel (HBC-RGD) was developed to enhance the adhesion and proliferation of BMSCs within the hydrogel. The successful synthesis of HBC-RGD was confirmed by FTIR and H NMR. Both HBC and HBC-RGD hydrogel had desired thermosensitivity, biocompatibility and enzymatic degradability in vitro. Compared with HBC hydrogel, HBC-RGD hydrogel was more beneficial for the adhesion and proliferation of BMSCs. Furthermore, the BMSCs incorporated HBC-RGD (BMSCs/HBC-RGD) hydrogel could inhibit the proliferation of keloid fibroblasts (Kfs) and suppress the nodular collagenous fibers of keloid tissue. These results suggested that the HBC-RGD hydrogel could be applied as a potential 3D hydrogel scaffold for cell culture, and BMSCs/HBC-RGD hydrogel was potential to be applied for keloid therapy with subcutaneous in-situ injection in the future.
骨髓间充质干细胞（BMSCs）的细胞治疗是许多疾病治疗的一种潜在方法，包括瘢痕疙瘩。在本研究中，开发了一种 Arg-Gly-Asp（RGD）修饰的羟丁基壳聚糖（HBC）水凝胶（HBC-RGD），以增强 BMSCs 在水凝胶中的黏附和增殖。通过傅里叶变换红外光谱（FTIR）和核磁共振（H NMR）证实了 HBC-RGD 的成功合成。HBC 和 HBC-RGD 水凝胶均具有理想的热响应性、生物相容性和体外酶降解性。与 HBC 水凝胶相比，HBC-RGD 水凝胶更有利于 BMSCs 的黏附和增殖。此外，负载 BMSCs 的 HBC-RGD（BMSCs/HBC-RGD）水凝胶可抑制瘢痕疙瘩成纤维细胞（Kfs）的增殖，并抑制瘢痕疙瘩组织的结节胶原纤维。这些结果表明，HBC-RGD 水凝胶可用作细胞培养的潜在 3D 水凝胶支架，并且 BMSCs/HBC-RGD 水凝胶有望在未来通过皮下原位注射用于瘢痕疙瘩治疗。
