Liu Feng-Jun, Dong Wen-Ya, Zhao Hao, Shi Xin-Huai, Zhang Yu-Ling
Animal College of Science and Technology, Henan University of Science and Technology, Luoyang, 471003, PR China.
Animal College of Science and Technology, Henan University of Science and Technology, Luoyang, 471003, PR China.
Theriogenology. 2019 Mar 1;126:49-54. doi: 10.1016/j.theriogenology.2018.12.002. Epub 2018 Dec 3.
Infertility is a serious public health problem worldwide. Molybdenum (Mo) plays an important role in maintaining normal metabolism. To explore the therapeutic efficacy of molybdenum (Mo) on male infertility, 90 mice were randomly divided into control, busulfan and busulfan + Mo groups. The male mice in the busulfan and busulfan + Mo groups were exposed to busulfan (20 mg/kg body weight) with a single intraperitoneal injection to establish the infertility model. The sterile mice were successfully obtained 30 days after busulfan exposure. Then, the male mice in the busulfan + Mo group were given drinking water containing 20 mg/L Mo continuously for 42 days. At 72 Day after treatment, 30 mice in the three groups were tested for various indices, and 60 mice were mated with females in spontaneous estrus. Mo significantly reversed the thinner seminiferous tubules and disappeared tubule and germ cells. Mo also normalized previously abnormal levels of testosterone, estradiol, luteinizing hormone, superoxide dismutase, lactate dehydrogenase, malondialdehyde. Furthermore, expression levels expression of Bax, Bcl-2, caspase-3 and caspase-9 returned to control levels; and finally, Mo-treated sterile mice obtained offspring with normal number and gender ratio. These results suggested that Mo at 20 mg/L had a significant therapeutic effect on reproductive dysfunction in sterile mice. Its mechanism could via repair of damaged testicular structures, regulation of abnormal reproductive hormone levels, decreased oxidative stress or and resistance to cell apoptosis. Mo may be a new candidate medicine for treatment of male infertility.
不孕不育是一个全球性的严重公共卫生问题。钼（Mo）在维持正常新陈代谢中起着重要作用。为了探究钼（Mo）对男性不育症的治疗效果，90只小鼠被随机分为对照组、白消安组和白消安+钼组。白消安组和白消安+钼组的雄性小鼠通过单次腹腔注射白消安（20mg/kg体重）来建立不育模型。在白消安暴露30天后成功获得不育小鼠。然后，白消安+钼组的雄性小鼠连续42天饮用含20mg/L钼的水。治疗后72天，对三组中的30只小鼠进行各项指标检测，另外60只小鼠与处于自然发情期的雌性小鼠交配。钼显著逆转了生精小管变薄以及小管和生殖细胞消失的情况。钼还使之前异常的睾酮、雌二醇、黄体生成素、超氧化物歧化酶、乳酸脱氢酶、丙二醛水平恢复正常。此外，Bax、Bcl-2、caspase-3和caspase-9的表达水平恢复到对照水平；最后，经钼治疗的不育小鼠获得了数量和性别比例正常的后代。这些结果表明，20mg/L的钼对不育小鼠的生殖功能障碍具有显著的治疗作用。其机制可能是通过修复受损的睾丸结构、调节异常的生殖激素水平、降低氧化应激或抵抗细胞凋亡。钼可能是一种治疗男性不育症的新型候选药物。
