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牡蛎肽对雷公藤内酯醇诱导的雄性小鼠睾丸损伤的潜在保护作用及可能机制。

The Potential Protective Effect and Possible Mechanism of Peptides from Oyster () Hydrolysate on Triptolide-Induced Testis Injury in Male Mice.

机构信息

College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.

Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China.

出版信息

Mar Drugs. 2021 Oct 9;19(10):566. doi: 10.3390/md19100566.

DOI:10.3390/md19100566
PMID:34677464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8539321/
Abstract

Peptides from oyster hydrolysate (OPs) have a variety of biological activities. However, its protective effect and exact mechanism on testicular injury remain poorly understood. This study aimed to evaluate the protective effect of OPs on triptolide (TP)-induced testis damage and spermatogenesis dysfunction and investigate its underlying mechanism. In this work, the TP-induced testis injury model was created while OPs were gavaged in mice for 4 weeks. The results showed that OPs significantly improved the sperm count and motility of mice, and alleviated the seminiferous tubule injury. Further study showed that OPs decreased malonaldehyde (MDA) level and increased antioxidant enzyme (SOD and GPH-Px) activities, attenuating oxidative stress and thereby reducing the number of apoptotic cells in the testis. In addition, OPs improved the activities of enzymes (LDH, ALP and ACP) related to energy metabolism in the testis and restored the serum hormone level of mice to normal. Furthermore, OPs promoted the expression of Nrf2 protein, and then increased the expression of antioxidant enzyme regulatory protein (HO-1 and NQO1) in the testis. OPs inhibited JNK phosphorylation and Bcl-2/Bax-mediated apoptosis. In conclusion, OPs have a protective effect on testicular injury and spermatogenesis disorders caused by TP, suggesting the potential protection of OPs on male reproduction.

摘要

牡蛎水解肽（OPs）具有多种生物活性。然而，其对睾丸损伤的保护作用及其确切机制仍知之甚少。本研究旨在评估 OPs 对雷公藤内酯醇（TP）诱导的睾丸损伤和生精功能障碍的保护作用，并探讨其潜在机制。在这项工作中，通过灌胃 OPs 4 周建立了 TP 诱导的睾丸损伤模型。结果表明，OPs 显著提高了小鼠的精子计数和活力，减轻了曲细精管损伤。进一步的研究表明，OPs 降低了丙二醛（MDA）水平，增加了抗氧化酶（SOD 和 GPH-Px）的活性，从而减轻了睾丸的氧化应激并减少了凋亡细胞的数量。此外，OPs 改善了睾丸中与能量代谢相关的酶（LDH、ALP 和 ACP）的活性，并使小鼠的血清激素水平恢复正常。此外，OPs 促进了 Nrf2 蛋白的表达，进而增加了睾丸中抗氧化酶调节蛋白（HO-1 和 NQO1）的表达。OPs 抑制了 JNK 的磷酸化以及 Bcl-2/Bax 介导的细胞凋亡。总之，OPs 对 TP 引起的睾丸损伤和生精障碍具有保护作用，提示 OPs 对男性生殖具有潜在的保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/d6dcf5549768/marinedrugs-19-00566-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/ac47f182c857/marinedrugs-19-00566-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/a1041e796fab/marinedrugs-19-00566-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/0ec2952a35bf/marinedrugs-19-00566-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/5b579e0a8c19/marinedrugs-19-00566-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/b18a74dec40c/marinedrugs-19-00566-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/25fd6e0e7853/marinedrugs-19-00566-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/0577cad113d3/marinedrugs-19-00566-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/250cc4a5766d/marinedrugs-19-00566-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/d6dcf5549768/marinedrugs-19-00566-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/ac47f182c857/marinedrugs-19-00566-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/a1041e796fab/marinedrugs-19-00566-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/0ec2952a35bf/marinedrugs-19-00566-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/5b579e0a8c19/marinedrugs-19-00566-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/b18a74dec40c/marinedrugs-19-00566-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/25fd6e0e7853/marinedrugs-19-00566-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/0577cad113d3/marinedrugs-19-00566-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/250cc4a5766d/marinedrugs-19-00566-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c0d/8539321/d6dcf5549768/marinedrugs-19-00566-g009.jpg

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