Li Yunlong, Liu Tong, Zhang Junzheng
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, 100094 Beijing, China.
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, 100094 Beijing, China
Biol Open. 2019 Jan 10;8(1):bio038984. doi: 10.1242/bio.038984.
The evolutionarily conserved Notch signaling pathway plays crucial roles in various developmental contexts. Multiple mechanisms are involved in the regulation of Notch pathway activity. Identified through a genetic mosaic screen, we show that the ATPase TER94 acts as a positive regulator of Notch signaling during wing development. Depletion of TER94 causes marginal notches in the adult wing and the reduction of Notch target genes and during wing margin formation. We provide evidence that TER94 is likely required for proper Notch protein localization and activation. Furthermore, we show that knockdown of the TER94 adaptor p47 leads to similar Notch signaling defects. Although the TER94 complex is implicated in various cellular processes, its role in the regulation of Notch pathways was previously uncharacterized. Our study demonstrates that TER94 positively regulates Notch signaling and thus reveals a novel role of TER94 in development.This article has an associated First Person interview with the first author of the paper.
进化上保守的Notch信号通路在各种发育环境中发挥着关键作用。Notch通路活性的调节涉及多种机制。通过遗传镶嵌筛选鉴定出，我们发现ATP酶TER94在翅发育过程中作为Notch信号的正向调节因子发挥作用。TER94的缺失导致成年翅出现边缘缺刻，并且在翅边缘形成过程中Notch靶基因减少。我们提供的证据表明，TER94可能是Notch蛋白正确定位和激活所必需的。此外，我们表明TER94衔接蛋白p47的敲低会导致类似的Notch信号缺陷。尽管TER94复合物参与了各种细胞过程，但其在Notch通路调节中的作用此前尚未得到描述。我们的研究表明TER94正向调节Notch信号，从而揭示了TER94在发育中的新作用。本文配有对该论文第一作者的相关第一人称访谈。
