Rödel W, Plaga W, Frank R, Knappe J
Institut für Biologische Chemie, Universität Heidelberg, Federal Republic of Germany.
Eur J Biochem. 1988 Oct 15;177(1):153-8. doi: 10.1111/j.1432-1033.1988.tb14356.x.
The structural gene of pyruvate formate-lyase (pfl) and that of pyruvate-formate-lyase-activating enzyme were shown to be adjacent on the chromosomal map of Escherichia coli. DNA sequencing was performed along a stretch of 3592 nucleotides to obtain the amino acid sequences of both proteins. The derived primary structures (759 and 245 residues) were confirmed by partial structure analyses on the purified proteins. The open reading frames are separated by a 194-nucleotide stretch, and their flanking regions include signal elements that are compatible with separate control of protein synthesis from the two genes.
丙酮酸甲酸裂解酶（pfl）的结构基因和丙酮酸甲酸裂解酶激活酶的结构基因在大肠杆菌的染色体图谱上显示是相邻的。沿着一段3592个核苷酸进行了DNA测序，以获得这两种蛋白质的氨基酸序列。通过对纯化蛋白质的部分结构分析证实了推导的一级结构（分别为759和245个残基）。开放阅读框被一段194个核苷酸的序列隔开，它们的侧翼区域包含与这两个基因蛋白质合成的独立控制相匹配的信号元件。
