Alatawi Fatema Suliman, Faridi Uzma A, Alatawi Mohsen Suliaman
Department of Biochemistry, College of Science, University of Tabuk, Tabuk, Saudi Arabia.
Departmment of Pediatric, College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Saudi Pharm J. 2018 Dec;26(8):1208-1213. doi: 10.1016/j.jsps.2018.07.012. Epub 2018 Jul 20.
In diabetes mellitus, uncontrolled hyperglycemia has been reported to induce oxidative stress, which may lead to health complications. Vitamin D, however, acts as a non-enzymatic antioxidant to protect cells against oxidative stress and damage.
To investigate the antioxidative effect of vitamin D combined with calcium in streptozotocin (STZ)-induced diabetic rats.
Rats were divided into four groups (ten rats in each group). The first group (control) received a normal diet and water. The second group, including STZ-induced diabetic rats (diabetic controls), received a normal diet and water. The third group, also including STZ-induced diabetic rats, received vitamin D (2000 IU/day) with calcium (500 mg/kg/day) orally for 28 consecutive days. The fourth group consisted of STZ-induced diabetic rats that received insulin treatment for 28 consecutive days. Activities of superoxide dismutase (SOD), glutathione peroxidase (GPO) and catalase were measured in the liver tissues. The level of malonaldehyde (MDA) was measured in the plasma.
Diabetic rats showed a significant decrease in the activities of SOD, GPO and catalase compared to normal rats. Oral administration of vitamin D with calcium to diabetic rats caused a significant increase in the activities of SOD, GPO and catalase compared with the untreated group. Furthermore, the plasma level of MDA was significantly elevated in diabetic rats compared to normal rats. Diabetic rats treated with vitamin D and calcium had a significantly reduced level of MDA, suggesting that vitamin D with calcium played a vital role in the protection of tissues from damage by free radicals.
Oral supplementation with vitamin D and calcium may be a useful treatment for diabetic patients to reduce/prevent the pathological complications of diabetes.
据报道，在糖尿病中，未得到控制的高血糖会引发氧化应激，这可能导致健康并发症。然而，维生素D作为一种非酶抗氧化剂，可保护细胞免受氧化应激和损伤。
探讨维生素D联合钙对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗氧化作用。
将大鼠分为四组（每组10只）。第一组（对照组）给予正常饮食和水。第二组，包括STZ诱导的糖尿病大鼠（糖尿病对照组），给予正常饮食和水。第三组，同样包括STZ诱导的糖尿病大鼠，连续28天口服维生素D（2000 IU/天）和钙（500 mg/kg/天）。第四组由连续28天接受胰岛素治疗的STZ诱导的糖尿病大鼠组成。测定肝组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPO）和过氧化氢酶的活性。测定血浆中丙二醛（MDA）的水平。
与正常大鼠相比，糖尿病大鼠的SOD、GPO和过氧化氢酶活性显著降低。糖尿病大鼠口服维生素D和钙后，与未治疗组相比，SOD、GPO和过氧化氢酶活性显著增加。此外，与正常大鼠相比，糖尿病大鼠血浆MDA水平显著升高。用维生素D和钙治疗的糖尿病大鼠MDA水平显著降低，表明维生素D联合钙在保护组织免受自由基损伤方面起着至关重要的作用。
口服补充维生素D和钙可能是糖尿病患者减少/预防糖尿病病理并发症的一种有效治疗方法。
