维生素 C 和 E 对链脲佐菌素诱导的糖尿病大鼠唾液腺氧化应激和抗氧化系统的影响。
Effect of vitamin C and E on oxidative stress and antioxidant system in the salivary glands of STZ-induced diabetic rats.
机构信息
Department of Biomaterials and Oral Biology, Faculdade de Odontologia, Universidade de São Paulo (USP), São Paulo, Brazil.
Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
出版信息
Arch Oral Biol. 2020 Aug;116:104765. doi: 10.1016/j.archoralbio.2020.104765. Epub 2020 May 16.
OBJECTIVE
We examined the effects of vitamin C and E supplementation in the prevention of oxidative stress in the salivary glands of STZ-induced diabetic rats.
DESIGN
Forty-eight male Wistar rats were divided into six groups (n = 8 in each): control (C), control supplemented with vitamin C (Cvc) and E (Cve), diabetic (D), and diabetic supplemented with vitamin C (Dvc) and E (Dve). Vitamin C (150 mg/kg) and E (300 mg/kg) were daily administered for 21 days. Serum ascorbic acid and α-tocopherol levels were quantified. Glandular levels of hydrogen peroxide (HO), superoxide anion (O), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), malondialdehyde (MDA) and the total antioxidant status (TAS) were estimated.
RESULTS
Vitamin C and E levels were reduced in D group. Vitamin C decreased the levels of O in the salivary gland of diabetic rats. Vitamin E increased the concentration of O in PA gland of diabetic animals. In the SM gland of the diabetic group, MDA, SOD, GPx and TAS increased. Dve presented reduced SOD activity and increased GR, GPx, and MDA. Dve increased GPx, Gr and TAS levels. In the PA gland, MDA, SOD, CAT, GPx, GR, and TAS were similar in C and D. TAS, SOD, CAT, GPx, and GR increased in Dvc. Vitamin E supplementation resulted in increased MDA and CAT levels and reduced SOD activity.
CONCLUSION
In the SM glands of the diabetic rats, vitamin C supplementation improved the antioxidant system, while vitamin E acted as pro-oxidant.
目的
研究维生素 C 和 E 补充剂对链脲佐菌素诱导的糖尿病大鼠唾液腺氧化应激的预防作用。
设计
将 48 只雄性 Wistar 大鼠分为六组（每组 8 只）：对照组（C）、维生素 C（Cvc）和 E（Cve）补充组、糖尿病组（D）和维生素 C（Dvc）和 E（Dve）补充组。每天给予维生素 C（150mg/kg）和 E（300mg/kg），共 21 天。测定血清抗坏血酸和 α-生育酚水平。估计腺体过氧化氢（HO）、超氧阴离子（O）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、过氧化氢酶（CAT）、丙二醛（MDA）和总抗氧化状态（TAS）的水平。
结果
D 组维生素 C 和 E 水平降低。维生素 C 降低了糖尿病大鼠唾液腺 O 的水平。维生素 E 增加了糖尿病动物 PA 腺体中 O 的浓度。在糖尿病组的 SM 腺体中，MDA、SOD、GPx 和 TAS 增加。Dve 表现出 SOD 活性降低和 GR、GPx、MDA 增加。Dve 增加了 GPx、Gr 和 TAS 水平。在 PA 腺体中，C 和 D 组的 MDA、SOD、CAT、GPx、GR 和 TAS 相似。Dvc 中 TAS、SOD、CAT、GPx、GR 和 Gr 增加。维生素 E 补充导致 MDA 和 CAT 水平升高，SOD 活性降低。
结论
在糖尿病大鼠的 SM 腺体中，维生素 C 补充剂改善了抗氧化系统，而维生素 E 则表现为促氧化剂。
Zotero 插件