胰岛素对正常及糖尿病大鼠循环淋巴细胞中丙酮酸脱氢酶的影响。

Effects of insulin on pyruvate dehydrogenase in circulating lymphocytes from normal and diabetic rats.

Rinaudo M T, Curto M, Bruno R, Marino C, Mostert M

Dipartimento di Medicina ed Oncologia Sperimentale, Facoltà di Medicina e Chirurgia, Università di Torino, Italy.

Int J Biochem. 1988;20(7):667-74. doi: 10.1016/0020-711x(88)90161-9.

Abstract

The in vivo and in vitro conditions which allow a response of rat circulating lymphocyte PDH to insulin are investigated. 2. In vivo tests show that inactive PDH (PDHi) prevails in diabetic rats and active PDH (PDHa) in hyperinsulinemic rats; in treated with insulin diabetic rats the PDHa/PDHi ratio (1.7) is similar to that of normal rats (PDHa/PDHi ratio = 2). 3. In vitro tests show a responsiveness of PDH to insulin only when 50 microM Ca2+ -Mg2+ and intact lymphocytes are used in the incubation medium. Insulin concentrations and contact time are important variables.

摘要

研究了使大鼠循环淋巴细胞丙酮酸脱氢酶（PDH）对胰岛素产生反应的体内和体外条件。2. 体内试验表明，无活性的PDH（PDHi）在糖尿病大鼠中占主导，而活性PDH（PDHa）在高胰岛素血症大鼠中占主导；用胰岛素治疗的糖尿病大鼠中，PDHa/PDHi比值（1.7）与正常大鼠相似（PDHa/PDHi比值 = 2）。3. 体外试验表明，只有当在孵育培养基中使用50微摩尔/升的Ca2+-Mg2+和完整淋巴细胞时，PDH才对胰岛素有反应。胰岛素浓度和接触时间是重要变量。