胰岛素对人循环淋巴细胞中丙酮酸脱氢酶的调节作用

Insulin modulation of pyruvate dehydrogenase in human circulating lymphocytes.

Curto M, Piccinini M, Bruno R, Mostert M, Rinaudo M T

Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Italia.

Int J Biochem. 1988;20(11):1211-7. doi: 10.1016/0020-711x(88)90221-2.

Abstract

In human circulating lymphocytes pyruvate dehydrogenase (PDH) complex is present in the active (PDHa) and inactive (PDHi) forms. 2. PDHi conversion into PDHa is stimulated when intact lymphocytes are incubated with 5 microU/ml insulin at pH 7.4, for 15 min at 37 degrees C in a medium supplemented with 50 microM Ca2+-Mg2+. 3. The generation of a mediator is strongly suggestive since a cell free preparation from circulating lymphocytes, treated as above described, still stimulates PDHi----PDHa conversion, when combined with either disrupted or intact lymphocytes.

摘要

在人循环淋巴细胞中，丙酮酸脱氢酶（PDH）复合体以活性形式（PDHa）和非活性形式（PDHi）存在。2. 当完整的淋巴细胞在pH 7.4、含有50 microM Ca2+-Mg2+的培养基中，于37℃与5微单位/毫升胰岛素孵育15分钟时，PDHi向PDHa的转化会受到刺激。3. 一种介质的产生具有强烈的暗示性，因为按照上述方法处理的循环淋巴细胞的无细胞制剂，在与破碎的或完整的淋巴细胞结合时，仍能刺激PDHi向PDHa的转化。