Gan Han Ming, Lee Melvin Vun Jye, Savka Michael A
Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
Deakin Genomics Centre, Deakin University, Geelong, Victoria, Australia.
Microbiol Resour Announc. 2018 Sep 6;7(9). doi: 10.1128/MRA.01045-18. eCollection 2018 Sep.
Using Illumina and Nanopore reads, we assembled a high-quality draft genome sequence of Allorhizobium vitis K309 (= ATCC 49767, = NCPPB 3554), a phytopathogenic strain isolated from a grapevine in Australia. The hybrid approach generated 50% fewer contigs and a 3-fold increase in the value compared with the previous Illumina-only assembly.
利用Illumina和Nanopore测序 reads，我们组装了葡萄根瘤菌K309（= ATCC 49767，= NCPPB 3554）的高质量基因组草图序列，该菌株是从澳大利亚一株葡萄藤中分离出的植物致病菌株。与之前仅使用Illumina的组装方法相比，这种混合方法产生的重叠群数量减少了50%，N50值提高了3倍。
