Programmable Biomaterials Laboratory (PBL), Institute of Materials (IMX)/Interfaculty Bioengineering Institute (IBI), School of Engineering (STI), École Polytechnique Federale de Lausanne (EPFL), MXC 340, Station 12, CH-1015, Lausanne, Switzerland.
Biomater Sci. 2019 Jan 29;7(2):532-541. doi: 10.1039/c8bm01249k.
DNA as a biomaterial has evoked great interest as a potential platform for therapeutics and diagnostics and as hydrogel scaffolds due to the relative ease of programming its robust and uniform shape, site-specific functionality and controlled responsive behavior. However, for a stable self-assembled product, a relatively high cation concentration is required to prevent denaturation. Physiological and cell-culture conditions do not match these concentrations and present additional nucleases that cause a serious threat to the integrity of DNA-based materials. For the translation of this promising technology towards bioengineering challenges, stability needs to be guaranteed. Over the past years, various methods have been developed addressing the stability-related weaknesses of DNA-origami. This mini-review explains the common stability issues and compares the stabilization strategies recently developed. We present a detailed overview of each method in order to ease the selection process on which method to use for future users of DNA-origami as a biomaterial.
DNA 作为一种生物材料，由于其相对容易编程其坚固而均匀的形状、特异性功能和可控的响应行为，因此作为治疗和诊断的潜在平台以及水凝胶支架而引起了极大的兴趣。然而，对于稳定的自组装产品，需要相对较高的阳离子浓度来防止变性。生理和细胞培养条件与这些浓度不匹配，并且存在额外的核酸酶，这对基于 DNA 的材料的完整性构成严重威胁。为了将这项有前途的技术转化为生物工程挑战，需要保证稳定性。在过去的几年中，已经开发了各种方法来解决 DNA 折纸术相关的稳定性弱点。这篇小型综述解释了常见的稳定性问题，并比较了最近开发的稳定化策略。我们详细介绍了每种方法，以便为未来的 DNA 折纸术作为生物材料的使用者选择使用哪种方法提供便利。
