Negara Ketut Surya, Suwiyoga Ketut, Pemayun Tjokorda Gede Astawa, Sudewi Anak Agung Raka, Astawa Nyoman Mantik, Arijana I Gusti Nyoman Kamasan, Tunas Ketut
Department of Obstetrics and Gynecology, Medical Faculty of Udayana University /Sanglah Hospital, Bali, Indonesia.
Department of Neurology, Medical Faculty of Udayana University/Sanglah Hospital, Bali, Indonesia.
Rev Bras Ginecol Obstet. 2018 Dec;40(12):733-739. doi: 10.1055/s-0038-1675611. Epub 2018 Dec 7.
To determine the role of caspase-3, apoptosis-inducing factor (AIF), and B-cell lymphoma-2 (Bcl-2) expressions in term premature rupture of membrane (PROM).
An analytic observational study with case-control design was conducted, involving 52 subjects (37-42 weeks of gestation) who were divided into 2 groups: 26 cases of term delivery with PROM, and 26 controls of term delivery without PROM. The expressions of caspase-3, AIF, and Bcl-2 in the amniotic membrane were determined by immunohistochemistry. Data were analyzed using the chi-squared test. The risk of PROM was expressed by odds ratio (OR).
There were no significant differences in age, parity and body mass index between the two groups (> 0.05). High caspase-3 and AIF expressions increased the risk of PROM 17.64 times (OR = 17.64; 95% CI = 4.44-70.07; = 0.001) and 9.45 times (OR = 9.45; 95% CI= 2.62-34.07; = 0.001), respectively, while low Bcl-2 expression increased 10.39 times (OR = 10.39; 95% CI = 2.73-39.56; = 0.001)the risk of PROM .
High caspase-3 and AIF expressions and low Bcl-2 expression were risk factors for term PROM. Caspase-dependent and independent pathways of apoptosis were involved in the mechanism of PROM in term pregnancy.
确定半胱天冬酶 -3、凋亡诱导因子（AIF）和B细胞淋巴瘤 -2（Bcl -2）的表达在足月胎膜早破（PROM）中的作用。
进行一项采用病例对照设计的分析性观察研究，纳入52名孕周为37 - 42周的受试者，分为两组：26例足月胎膜早破分娩病例，以及26例足月未发生胎膜早破分娩的对照。通过免疫组织化学法测定羊膜中半胱天冬酶 -3、AIF和Bcl -2的表达。数据采用卡方检验进行分析。胎膜早破的风险用比值比（OR）表示。
两组在年龄、产次和体重指数方面无显著差异（> 0.05）。高半胱天冬酶 -3和AIF表达分别使胎膜早破风险增加17.64倍（OR = 17.64；95%可信区间 = 4.44 - 70.07；P = 0.001）和9.45倍（OR = 9.45；95%可信区间 = 2.62 - 34.07；P = 0.001），而低Bcl -2表达使胎膜早破风险增加10.39倍（OR = 10.39；95%可信区间 = 2.73 - 39.56；P = 0.001）。
高半胱天冬酶 -3和AIF表达以及低Bcl -2表达是足月胎膜早破的危险因素。凋亡的半胱天冬酶依赖性和非依赖性途径参与了足月妊娠胎膜早破的机制。
