克隆释放因子基因的遗传筛选。
Genetic screen for cloned release factor genes.
作者信息
Weiss R B, Murphy J P, Gallant J A
出版信息
J Bacteriol. 1984 Apr;158(1):362-4. doi: 10.1128/jb.158.1.362-364.1984.
Abstract
Nonsense suppression reflects competition between a nonsense suppressor tRNA and a translational release factor. This provides a simple way to screen for release factor genes cloned into a multicopy plasmid. We have confirmed that the expected competition occurs with the gene for release factor 2, cloned by C.T. Caskey et al. (C.T. Caskey, W.C. Forrester, W. Tate, and C.D. Ward, J. Bacteriol. 158:365-368, 1984), and used it to clone the gene for release factor 1.
摘要
无义抑制反映了无义抑制tRNA与翻译释放因子之间的竞争。这为筛选克隆到多拷贝质粒中的释放因子基因提供了一种简单方法。我们已经证实，C.T. 卡斯基等人（C.T. 卡斯基、W.C. 福雷斯特、W. 泰特和C.D. 沃德，《细菌学杂志》158:365 - 368，1984年）克隆的释放因子2基因会发生预期的竞争，并利用这一点克隆了释放因子1的基因。
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