Karci Bilge, Sokmen Kevser
Department of Periodontology, Faculty of Dentistry, Alanya Alaaddin Keykubat University, 07400 Alanya, Türkiye.
Biomedicines. 2025 May 9;13(5):1159. doi: 10.3390/biomedicines13051159.
: This study aimed to investigate endocan (ESM-1) levels in periodontitis patients before and after non-surgical periodontal treatment by analyzing the relationship between vascular endothelial growth factor A (VEGF-A) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF). : This study included 26 periodontally healthy people as controls (Group 1) and 27 patients with Stage III-Grade B periodontitis (Group 2). Demographic and periodontal variables were assessed. GCF samples were collected from every subject both before and 6 weeks following non-surgical periodontal therapy (NSPT). Using an enzyme-linked immunosorbent test, biomarker levels were determined. : The periodontitis patients showed higher ESM-1 levels than the controls, although the difference was not significant ( > 0.005). The ESM-1 levels decreased significantly after treatment ( = 0.001). The VEGF-A levels did not differ significantly between the periodontitis patients and controls ( > 0.005) and decreased non-significantly following treatment ( > 0.005). The TNF-α levels were significantly higher in the periodontitis patients than the controls ( = 0.000) and decreased significantly after treatment ( = 0.000). A significant correlation was found between TNF-α and both probing depth (PD) and interproximal clinical attachment level (iCAL) in the control group ( < 0.05). In the periodontitis group, the VEGF levels were significantly correlated with the gingival index (GI) ( < 0.05). Significant correlations were identified between ESM-1 and VEGF-A and ESM-1 and TNF-α, as well as VEGF-A and TNF-α, in both the control group and following treatment ( < 0.05). : ESM-1 and TNF-α levels decreased with non-surgical periodontal treatment in GCF. Within the limits of the study, the findings suggest that ESM-1 levels in periodontal tissues may be an indicator of periodontal disease.
本研究旨在通过分析龈沟液（GCF）中血管内皮生长因子A（VEGF-A）与肿瘤坏死因子-α（TNF-α）之间的关系，调查非手术牙周治疗前后牙周炎患者体内内源性血管生成素（ESM-1）水平。本研究纳入26名牙周健康者作为对照组（第1组）和27名III-B期牙周炎患者（第2组）。评估人口统计学和牙周变量。在非手术牙周治疗（NSPT）前及治疗后6周，从每位受试者采集GCF样本。采用酶联免疫吸附试验测定生物标志物水平。牙周炎患者的ESM-1水平高于对照组，尽管差异无统计学意义（P>0.005）。治疗后ESM-1水平显著降低（P=0.001）。牙周炎患者与对照组之间的VEGF-A水平差异无统计学意义（P>0.005），治疗后无显著下降（P>0.005）。牙周炎患者的TNF-α水平显著高于对照组（P=0.000），治疗后显著降低（P=0.000）。对照组中，TNF-α与探诊深度（PD）和邻面临床附着水平（iCAL）均显著相关（P<0.05）。在牙周炎组中，VEGF水平与牙龈指数（GI）显著相关（P<0.05）。对照组及治疗后，ESM-1与VEGF-A、ESM-1与TNF-α以及VEGF-A与TNF-α之间均存在显著相关性（P<0.05）。GCF中，非手术牙周治疗后ESM-1和TNF-α水平降低。在本研究范围内，研究结果表明牙周组织中ESM-1水平可能是牙周疾病的一个指标。
