Zhang Renzhou, Yang Songtao, Zhang Wei, Zhang Tao, Xie Zhijing, Feng Hao, Wang Shujun, Xia Xianzhu
Laboratory of Virology, Institute of Military Veterinary, Academy of Military Medical Sciences, 130062 Changchun, Jilin Province, China.
Virus Genes. 2010 Jun;40(3):397-402. doi: 10.1007/s11262-010-0466-7. Epub 2010 Mar 9.
Sequences of the full-length gene encoding the main capsid protein VP2 of 22 strains of canine parvovirus (CPV), isolated from domestic dogs in China between 1983 and 2008, were determined and analyzed in comparison with the sequences of 30 other strains of CPV from China and reference CPV isolates retrieved from GenBank. Three types of CPV, including CPV-2, CPV-2a, and CPV-2b, were detected, and CPV-2a (with 297-Ala mutation) was predominant in China. The unique Ile-324 mutation in the VP2 of Chinese CPV isolates was detected, as compared with a Tyr-324 in the VP2 of the reference CPV strains. A phylogenetic tree constructed from the VP2 genes showed that most of the Chinese strains classified in a cluster consisting of Chinese and Korean field isolates, which were distinct from Thai, U.S., and Italian isolates.
对1983年至2008年间从中国家犬中分离出的22株犬细小病毒（CPV）主要衣壳蛋白VP2的全长基因序列进行了测定和分析，并与来自中国的其他30株CPV序列以及从GenBank检索到的CPV参考分离株序列进行了比较。检测到三种类型的CPV，包括CPV-2、CPV-2a和CPV-2b，其中CPV-2a（具有297-Ala突变）在中国占主导地位。与参考CPV株VP2中的Tyr-324相比，在中国CPV分离株的VP2中检测到独特的Ile-324突变。由VP2基因构建的系统发育树表明，大多数中国菌株分类在一个由中国和韩国野外分离株组成的簇中，这与泰国、美国和意大利的分离株不同。
