Department of Life Sciences, University of Modena and Reggio Emilia, 41125, Modena, Italy.
Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, 64100, MO, USA.
Proteomics. 2019 Feb;19(4):e1800314. doi: 10.1002/pmic.201800314. Epub 2019 Jan 23.
Pomacea canaliculata is a freshwater snail with interesting biological features that include invasiveness, human parasite hosting, and adult regeneration. Its immune system may represent the target for strategies aimed at controlling the spread of the snail population and its hosting of the human parasite Angiostrongylus cantonensis. Moreover, immune functions likely have a role in the snail's ability to wound heal and regenerate. Despite its importance in multiple processes, very little is known about the molecular basis of P. canaliculata immunity. Aiming to contribute to filling this gap, the ultrastructure of circulating hemocytes in healthy snails is studied and the first proteomic analysis of these cells is performed, evidencing 83 unique proteins, 96% of which have identifiable homologs in other species. Fifteen proteins are retrieved as potentially involved in immune-related signaling pathways, such as hemocyanin, C1q-like protein, and HSP90 together with cytoskeleton and cytoskeleton-related proteins involved in cell motility and membrane dynamics. This first proteome study on non-stimulated hemocytes provides a valid reference for future investigations on the molecular changes under stressful circumstances, like pathogen exposure, wounding, or environmental changes.
Pomacea canaliculata 是一种淡水蜗牛，具有有趣的生物学特征，包括入侵性、人类寄生虫宿主和成年再生。它的免疫系统可能是控制蜗牛种群传播及其宿主 Angiostrongylus cantonensis 的策略的目标。此外，免疫功能可能在蜗牛伤口愈合和再生的能力中发挥作用。尽管它在多个过程中很重要，但对 P. canaliculata 免疫的分子基础知之甚少。为了弥补这一空白，研究了健康蜗牛循环血细胞的超微结构，并对这些细胞进行了首次蛋白质组分析，证明了 83 种独特的蛋白质，其中 96%在其他物种中具有可识别的同源物。有 15 种蛋白质被认为可能参与免疫相关的信号通路，如血蓝蛋白、C1q 样蛋白和 HSP90 以及参与细胞运动和膜动力学的细胞骨架和细胞骨架相关蛋白。这项对非刺激血细胞的首次蛋白质组研究为未来在病原体暴露、创伤或环境变化等应激情况下进行分子变化的研究提供了有效的参考。
