McLaughlin G L, Deloron P, Huong A Y, Sezibera C, Campbell G H
Malaria Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1988 Sep;26(9):1704-7. doi: 10.1128/jcm.26.9.1704-1707.1988.
We studied the accuracy of PFR1-AP, a synthetic DNA hybridization probe conjugated to alkaline phosphatase, in monitoring Plasmodium falciparum parasitemia during in vivo drug susceptibility surveys. Duplicate blood samples were collected from six children enrolled in a 14-day in vivo chloroquine study in Rwanda. Results obtained by microscopic examination of Giemsa-stained thick blood smears and by DNA hybridization were compared. Both techniques successfully monitored an infection with chloroquine-susceptible parasites and infections exhibiting various levels of resistance to treatment. For each patient, temporal evolution of the microscopic parasite counts and the DNA hybridization signals were closely parallel, although a wide range of rapidly changing levels of parasitemia occurred through the course of the study. This suggests that DNA hybridization assay using PFR1-AP detects P. falciparum parasites sensitively and specifically and is a valuable tool for drug resistance surveys.
我们研究了与碱性磷酸酶偶联的合成DNA杂交探针PFR1-AP在体内药物敏感性调查期间监测恶性疟原虫血症的准确性。从卢旺达一项为期14天的体内氯喹研究中的六名儿童采集了重复的血样。比较了通过吉姆萨染色厚血涂片显微镜检查和DNA杂交获得的结果。两种技术都成功监测了对氯喹敏感的寄生虫感染以及表现出不同程度治疗抗性的感染。对于每位患者，显微镜下寄生虫计数和DNA杂交信号的时间演变密切平行，尽管在研究过程中出现了广泛的快速变化的疟原虫血症水平。这表明使用PFR1-AP的DNA杂交测定法能够灵敏且特异地检测恶性疟原虫，是耐药性调查的一种有价值的工具。
