利用分子克隆DNA探针特异性检测恶性疟原虫疟疾

Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe.

作者信息

Guntaka R V, Rao A S, Green T J, Collins W E

出版信息

Biochem Biophys Res Commun. 1986 Jul 16;138(1):363-8. doi: 10.1016/0006-291x(86)90289-5.

Abstract

A highly repeated DNA sequence from Plasmodium falciparum was cloned and used as a probe in molecular hybridization to detect malaria. Our results indicate that the probe is specific to P. falciparum but not to other species of Plasmodium and is extremely sensitive. As little as a 20 pg parasite DNA, which is equivalent to about 1000 parasites can be detected. The cloned DNA can be used as a diagnostic tool to follow the course of infection of falciparum malaria.

摘要

恶性疟原虫的一段高度重复DNA序列被克隆，并用作分子杂交中的探针来检测疟疾。我们的结果表明，该探针对恶性疟原虫具有特异性，对其他疟原虫物种则无特异性，且极其灵敏。低至20皮克的寄生虫DNA（相当于约1000个寄生虫）都能被检测到。克隆的DNA可作为一种诊断工具来追踪恶性疟原虫感染的病程。