Holmberg M, Björkman A, Franzén L, Aslund L, Lebbad M, Pettersson U, Wigzell H
Bull World Health Organ. 1986;64(4):579-85.
The spot hybridization assay for the detection of Plasmodium falciparum reported here uses as probe a repetitive DNA sequence from this species and exhibits a high degree of species specificity. Isolates from African, Asian, and South American patients were positive in the assay and gametocytes could be detected at the same level of parasitaemia as asexual parasites. An RNA probe containing the same repetitive sequence as the DNA probe has a detection limit of 1 parasite per 10(6) red blood cells. Comparison of the results of the assay with those obtained by microscopic examination of blood films indicated that the assay was more sensitive than microscopy if the blood films were examined for only 10 minutes; however, 40 minutes' examination by microscopy was slightly more sensitive than the assay.
本文报道的用于检测恶性疟原虫的斑点杂交试验，使用该物种的一个重复DNA序列作为探针，具有高度的物种特异性。来自非洲、亚洲和南美洲患者的分离株在该试验中呈阳性，并且在与无性寄生虫相同的寄生虫血症水平下可以检测到配子体。含有与DNA探针相同重复序列的RNA探针的检测限为每10⁶个红细胞中有1个寄生虫。将该试验结果与通过显微镜检查血涂片获得的结果进行比较表明，如果血涂片仅检查10分钟，该试验比显微镜检查更敏感；然而，显微镜检查40分钟比该试验略敏感。
