恶性疟原虫基因组中的高度重复非编码序列由21个碱基对的串联重复序列组成。

Highly reiterated non-coding sequence in the genome of Plasmodium falciparum is composed of 21 base-pair tandem repeats.

作者信息

Aslund L, Franzén L, Westin G, Persson T, Wigzell H, Pettersson U

出版信息

J Mol Biol. 1985 Oct 5;185(3):509-16. doi: 10.1016/0022-2836(85)90067-1.

Abstract

Clones containing highly reiterated DNA sequences were isolated from a Plasmodium falciparum genomic library. One clone, Rep2, was selected for further characterization by nucleotide sequence analysis. The results revealed that the insert of this clone is composed of tandemly arranged 21 base-pair imperfect repeats. These repeats are estimated to comprise approximately 1% of the P. falciparum genome and there are 10(4) to 2 X 10(5) copies, depending on the genome size estimate used for calculation. Moreover, the repeats are organized in clusters and do not appear to be transcribed in non-synchronized P. falciparum cultures.

摘要

从恶性疟原虫基因组文库中分离出含有高度重复DNA序列的克隆。选择了一个名为Rep2的克隆进行核苷酸序列分析以进一步表征。结果显示，该克隆的插入片段由串联排列的21个碱基对的不完全重复序列组成。据估计，这些重复序列约占恶性疟原虫基因组的1%，根据用于计算的基因组大小估计，其拷贝数为10⁴至2×10⁵个。此外，这些重复序列成簇组织，在未同步化的恶性疟原虫培养物中似乎不被转录。